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Comprehensive comparison on the anti-inflammatory effects of three species of Sigesbeckia plants based on NF-κB and MAPKs signal pathways in vitro
Linghu,Ke Gang1; Zhao,Guan Ding1; Xiong,Wei1; Sang,Wei1; Xiong,Shi Hang1; Tse,Anfernee Kai Wing4; Hu,Yuanjia1; Bian,Zhaoxiang3; Wang,Yitao1; Yu,Hua1,2,3
2020-03-25
Source PublicationJournal of Ethnopharmacology
ISSN0378-8741
Volume250
Abstract

Ethnopharmacological relevance: Sigesbeckiae Herba (SH), a traditional anti-inflammatory Chinese herbal medicine, is originated from the plants of Sigesbeckia pubescens Makino (SP), S. orientalis L. (SO) and S. glabrescens Makino (SG). The current studies reported that the chemical constituents in the three species of plants were different. Aim of the study: The aim of this study is to provide a systemic comparison on the anti-inflammatory effects and the underlying molecular mechanisms among the three plants based on their effects on nuclear factor-κB (NF-κB) and mitogen-activated protein kinases (MAPKs) signal pathways in vitro. Material and methods: Twenty-four batches of three Sigesbeckia herbs were collected from different regions of China and extracted with 50% ethanol. The distribution of 6 compounds in the 24 batches of SH extracts were characterized by UPLC analysis. The cytotoxicity of all extracts to RAW264.7 cells in the absence or presence of lipopolysaccharide (LPS) were examined by 3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The anti-inflammatory effects of the extracts were investigated using Griess reagent and enzyme-linked immunosorbent assay. The underlying mechanisms of the representative samples (SP007, SO005 and SG003) for individual species were examined by western blotting and immunofluorescence staining. Results: The estimated average sub-lethal dose (LD) of SP, SO and SG on RAW264.7 cells were 181.7 ± 15.7, 291.5 ± 33.9 and 317.1 ± 16.3 μg/mL, respectively. In LPS-stimulated RAW264.7 cells, the inhibitory effects of SH species were determined to be SP > SO > SG on NO release, while SP ~ SO > SG on secretion of post-inflammatory cytokines (TNF-α, IL-6 and MCP-1). Moreover, suppression on LPS-induced excessive expressions of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), as well as the activation of NF-κB and phosphorylation of MAPKs were investigated to be associated to the anti-inflammatory effects for all SH species. Conclusions: We firstly reported a systemic comparison on the anti-inflammatory properties for the three main plant origins of SH. Although SG showed lower toxicity and less anti-inflammatory effects compared with SP and SO in LPS-induced RAW264.7 cells, comparable inhibitory effects on NF-κB and MAPKs pathways and the reduction of LPS-induced iNOS and COX-2 were observed in the anti-inflammatory process for all Sigesbeckia plants.

KeywordAnti-inflammation Mapks Nf-κb Sigesbeckia Glabrescens Makino Sigesbeckia OrientaL.s L. Sigesbeckia Pubescens Makino
DOI10.1016/j.jep.2019.112530
URLView the original
Indexed BySCIE
WOS Research AreaPlant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
WOS SubjectPlant Sciences ; Chemistry, Medicinal ; Integrative & Complementary Medicine ; Pharmacology & Pharmacy
WOS IDWOS:000510954100042
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Cited Times [WOS]:5   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorYu,Hua
Affiliation1.Institute of Chinese Medical Sciences,State Key Laboratory of Quality Research in Chinese Medicine,University of Macau,China
2.HKBU Shenzhen Research Center,Shenzhen,China
3.School of Chinese Medicine,Hong Kong Baptist University,Kowloon Tong,Hong Kong
4.Programme of Food Science and Technology,Division of Science and Technology,Beijing Normal University-Hong Kong Baptist University United International College,Zhuhai,China
First Author AffilicationInstitute of Chinese Medical Sciences
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Linghu,Ke Gang,Zhao,Guan Ding,Xiong,Wei,et al. Comprehensive comparison on the anti-inflammatory effects of three species of Sigesbeckia plants based on NF-κB and MAPKs signal pathways in vitro[J]. Journal of Ethnopharmacology,2020,250.
APA Linghu,Ke Gang,Zhao,Guan Ding,Xiong,Wei,Sang,Wei,Xiong,Shi Hang,Tse,Anfernee Kai Wing,Hu,Yuanjia,Bian,Zhaoxiang,Wang,Yitao,&Yu,Hua.(2020).Comprehensive comparison on the anti-inflammatory effects of three species of Sigesbeckia plants based on NF-κB and MAPKs signal pathways in vitro.Journal of Ethnopharmacology,250.
MLA Linghu,Ke Gang,et al."Comprehensive comparison on the anti-inflammatory effects of three species of Sigesbeckia plants based on NF-κB and MAPKs signal pathways in vitro".Journal of Ethnopharmacology 250(2020).
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