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Cryptotanshinone protects dextran sulfate sodium-induced experimental ulcerative colitis in mice by inhibiting intestinal inflammation
Min,Xiangjing1; Zeng,Xi2; Zhao,Wenwen2; Han,Zhiwu3; Wang,Ying4; Han,Yantao2; Pei,Lixia5; Chen,Xiuping1,2,4
2020-10-01
Source PublicationPhytotherapy Research
ISSN0951-418X
Volume34Issue:10Pages:2639-2648
AbstractThe incidence of ulcerative colitis (UC) is increasing in recent years. The protective effect of cryptotanshinone, a natural compound from Salvia miltiorrhiza Bunge, on UC was investigated both in vivo and in vitro models. UC model was established by dextran sulfate sodium administration in drinking water and cryptotanshinone was orally administrated. RAW264.7 cells were stimulated by lipopolysaccharide (LPS) with or without cryptotanshinone pretreatment. The body weights and disease activity index (DAI) were recorded. The pathological alterations were evaluated by H&E staining. The levels of pro-inflammatory cytokines in colon tissues and cell culture medium were determined with enzyme-linked immune sorbent assay (ELISA) kits. The protein expression was detected by Western blotting and immunohistochemistry. Results showed that cryptotanshinone significantly increased the body weight and colon length, reduced the score of DAI, and improved pathological changes. Furthermore, the expression of inducible nitric oxide synthase, cyclooxygenase-2, receptor-interacting protein kinase 3, NF-κB p65 and the secretion of tumor necrosis factor-α, IL-6 in colon tissues and LPS-stimulated cells were significantly inhibited by cryptotanshinone. Besides, cryptotanshinone significantly inhibited LPS-triggered toll-like receptor 4 luciferase reporter activity with an IC at 7.2 μM. In conclusion, cryptotanshinone ameliorated experimental UC possibly by inhibiting intestinal inflammation.
Keywordcryptotanshinone inflammation ulcerative colitis
DOI10.1002/ptr.6693
URLView the original
Language英语
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Cited Times [WOS]:1   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorChen,Xiuping
Affiliation1.Key Lab for Pharmacology of Ministry of Education,Department of Pharmacology,Zunyi Medical University,Zunyi,China
2.Medical College,Qingdao University,Qingdao,China
3.Department of Pharmacy,The Affiliated Hospital of Qingdao University,Qingdao,China
4.State Key Laboratory of Quality Research in Chinese Medicine,Institute of Chinese Medical Sciences,University of Macau,Macao
5.Longhua Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai,China
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Min,Xiangjing,Zeng,Xi,Zhao,Wenwen,et al. Cryptotanshinone protects dextran sulfate sodium-induced experimental ulcerative colitis in mice by inhibiting intestinal inflammation[J]. Phytotherapy Research,2020,34(10):2639-2648.
APA Min,Xiangjing,Zeng,Xi,Zhao,Wenwen,Han,Zhiwu,Wang,Ying,Han,Yantao,Pei,Lixia,&Chen,Xiuping.(2020).Cryptotanshinone protects dextran sulfate sodium-induced experimental ulcerative colitis in mice by inhibiting intestinal inflammation.Phytotherapy Research,34(10),2639-2648.
MLA Min,Xiangjing,et al."Cryptotanshinone protects dextran sulfate sodium-induced experimental ulcerative colitis in mice by inhibiting intestinal inflammation".Phytotherapy Research 34.10(2020):2639-2648.
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