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Oligosaccharide-marker approach for qualitative and quantitative analysis of specific polysaccharide in herb formula by ultra-high-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry: Dendrobium officinale, a case study
Wong,Tin Long1; Li,Li Feng1; Zhang,Ji Xia2; Bai,Su Ping2; Zhou,Li Shuang1; Fung,Hau Yee1; Zhang,Quan Wei1; Ma,Dik Lung3; Leung,Chung Hang4; Zhao,Zhong Zhen1; Han,Quan Bin1
2019-12-06
Source PublicationJournal of Chromatography A
ISSN0021-9673
Volume1607
AbstractQualitative and quantitative analysis of polysaccharides in herb formula remain challenge due to the limited choices of analytical methods concerning the intrinsic characteristics of large molecular mass. Herein, an oligosaccharide-marker approach was newly developed for quality assessment of polysaccharides in herbal materials, using Dendrobium officinale as a case study. This method involved partial acid hydrolysis of D. officinale polysaccharide (DOP) followed by p-aminobenzoic ethyl ester (ABEE) derivatization. Two ABEE-labeled oligosaccharides namely, Te-Man-ABEE and Pen-Man-ABEE, were selected as chemical markers due to their high specificity in herb formula. The linear relationship between the content of these two markers and the content of DOP was then successfully established respectively. The linear relationship was further transformed to that between peak area of chemical markers and DOP content so that chemical markers were not necessary to be isolated for analysis. This linear relationship was systemically validated in terms of precision and accuracy. The results showed that these two oligosaccharide-markers presented a good linear relationship with DOP (R ≥ 0.997) in the range of 0.68–16.02 μg. These markers also demonstrated satisfactory precision (RSD < 7.0%), and recovery (91.41%–118.30%) in real sample determination. Additionally, there was no significant difference between the results given by the two chemical markers as the RSD values were not more than 7.0%. While concerning the results given by the oligosaccharide-markers and the previously-published polysaccharide marker, the RSD value was not more than 6.4%. These suggest that the oligosaccharide-marker approach is a simple, quick, and reliable method to qualitatively and quantitatively determine of specific polysaccharide in herb formula.
KeywordABEE-labeled oligosaccharide-marker Dendrobiumofficinale Polysaccharide Qualitative and quantitative analysis
DOI10.1016/j.chroma.2019.460388
URLView the original
Language英语
Scopus ID2-s2.0-85071336965
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Cited Times [WOS]:4   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.School of Chinese Medicine,Hong Kong Baptist University,Hong Kong,Hong Kong
2.School of Pharmacy,Xinxiang Medical University,Xinxiang,China
3.Department of Chemistry,Hong Kong Baptist University,Hong Kong,Hong Kong
4.State Key Laboratory of Quality Research in Chinese Medicine,Institute of Chinese Medical Sciences,University of Macau,Macao,China
Recommended Citation
GB/T 7714
Wong,Tin Long,Li,Li Feng,Zhang,Ji Xia,et al. Oligosaccharide-marker approach for qualitative and quantitative analysis of specific polysaccharide in herb formula by ultra-high-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry: Dendrobium officinale, a case study[J]. Journal of Chromatography A,2019,1607.
APA Wong,Tin Long,Li,Li Feng,Zhang,Ji Xia,Bai,Su Ping,Zhou,Li Shuang,Fung,Hau Yee,Zhang,Quan Wei,Ma,Dik Lung,Leung,Chung Hang,Zhao,Zhong Zhen,&Han,Quan Bin.(2019).Oligosaccharide-marker approach for qualitative and quantitative analysis of specific polysaccharide in herb formula by ultra-high-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry: Dendrobium officinale, a case study.Journal of Chromatography A,1607.
MLA Wong,Tin Long,et al."Oligosaccharide-marker approach for qualitative and quantitative analysis of specific polysaccharide in herb formula by ultra-high-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry: Dendrobium officinale, a case study".Journal of Chromatography A 1607(2019).
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