UM
Purified Astaxanthin from Haematococcus pluvialis Promotes Tissue Regeneration by Reducing Oxidative Stress and the Secretion of Collagen in Vitro and in Vivo
Chou,Hsin Yu1,9; Ma,Dik Lung3; Leung,Chung Hang4,10; Chiu,Chien Chih5,6,7,8; Hour,Tzyh Chyuan2; Wang,Hui Min David1
2020
Source PublicationOxidative Medicine and Cellular Longevity
ISSN1942-0900
Volume2020
AbstractIntracellular reactive apoptosis and reactive oxygen species (ROS) play a crucial role in ultraviolet- (UV-) induced inflammation and aging reaction in human dermal tissues. This study determines the mechanism by which Haematococcus pluvialis extracts (HPE) and purified astaxanthin (HPA) to promote skin regeneration in the injured tissue in vitro and in vivo. The results show that HPE and HPA decrease the DNA damage and promote the secretion of collagen from the human normal fibroblast cell line (Hs68) in a dose-dependent manner. UV irradiation and HPA reduce oxidative stress damage due to phorbol-12-myristate-13-acetate (PMA). When skin cells are injured by free radicals, cells undergo a programmed cellular death. Cellular apoptotic death is determined using annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) double staining to verify that there is no cell membrane asymmetry and that the nuclear membrane is broken. Inflammatory symptoms and apoptotic injuries to experimental rats in a group that is treated with HPA treated are decreased in a dose-dependent manner after UVB exposure (300 mJ/cm2) for 15 min in vivo, compared to the vehicle control group. These positive results show that HPA repairs UVB-triggered skin tissue injury and aging by conducting electrons out of cells to maintain a low level of oxidative stress so that collagen is synthesized in vitro and in vivo.
DOI10.1155/2020/4946902
URLView the original
Language英语
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Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.Program in Tissue Engineering and Regenerative Medicine,National Chung Hsing University,Taichung,402,Taiwan
2.Division of Biochemistry and Molecular Biology,Graduate Institute of Medicine,College of Medicine,Kaohsiung Medical University,Kaohsiung,807,Taiwan
3.Department of Chemistry,Hong Kong Baptist University,Kowloon Tong,Hong Kong,Hong Kong
4.State Key Laboratory of Quality Research in Chinese Medicine,Institute of Chinese Medical Sciences,University of Macau,Macao
5.Department of Biotechnology,Kaohsiung Medical University,Kaohsiung City,807,Taiwan
6.Department of Medical Research,Kaohsiung Medical University Hospital,Kaohsiung City,807,Taiwan
7.Graduate Institute of Biomedical Engineering,National Chung Hsing University,Taichung,402,Taiwan
8.Department of Medical Laboratory Science and Biotechnology,China Medical University,Taichung City,404,Taiwan
9.Graduate Institute of Medicine,College of Medicine,Kaohsiung Medical University,Kaohsiung City,807,Taiwan
10.College of Food and Biological Engineering,Jimei University,Xiamen,361021,China
Recommended Citation
GB/T 7714
Chou,Hsin Yu,Ma,Dik Lung,Leung,Chung Hang,et al. Purified Astaxanthin from Haematococcus pluvialis Promotes Tissue Regeneration by Reducing Oxidative Stress and the Secretion of Collagen in Vitro and in Vivo[J]. Oxidative Medicine and Cellular Longevity,2020,2020.
APA Chou,Hsin Yu,Ma,Dik Lung,Leung,Chung Hang,Chiu,Chien Chih,Hour,Tzyh Chyuan,&Wang,Hui Min David.(2020).Purified Astaxanthin from Haematococcus pluvialis Promotes Tissue Regeneration by Reducing Oxidative Stress and the Secretion of Collagen in Vitro and in Vivo.Oxidative Medicine and Cellular Longevity,2020.
MLA Chou,Hsin Yu,et al."Purified Astaxanthin from Haematococcus pluvialis Promotes Tissue Regeneration by Reducing Oxidative Stress and the Secretion of Collagen in Vitro and in Vivo".Oxidative Medicine and Cellular Longevity 2020(2020).
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