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An oligosaccharide-marker approach to quantify specific polysaccharides in herbal formula by LC-qTOF-MS: Danggui Buxue Tang, a case study
Li,Li Feng1; Wong,Tin Long1; Zhang,Ji Xia2; Zhou,Li Shuang1; Bai,Su Ping2; Fung,Hau Yee1; Cheng,Hui Yuan1; Zhang,Quan Wei1; Zheng,Hong Ming1; Bao,Wan Rong1; Ma,Dik Lung3; Leung,Chung Hang4; Zhang,Ge1; Bian,Zhao Xiang1; Lyu,Ai Ping1; Han,Quan Bin1
2020-06-05
Source PublicationJournal of Pharmaceutical and Biomedical Analysis
ISSN0731-7085
Volume185
AbstractPolysaccharides have broad bioactivities and are major components of water decoction of herb formulae. However, the quality control of polysaccharides remains a challenge. Oligosaccharide-fragment approach has been considered in elucidating chemical structures of polysaccharides, but never been used for quantitation. Using reference chemicals and a real sample Danggui Buxue Tang (DBT) in this study, an oligosaccharide-marker approach was established to quantify specific polysaccharides. Firstly, linear relationships between parent polysaccharides and hydrolysis-produced daughter oligosaccharides were verified using reference polysaccharides. Then in case of DBT, two fluorescence-labeled oligosaccharides with high specificity to individual parent polysaccharides were selected as markers. They were easily isolated and identified. Their potential in quantification of parent polysaccharides were satisfactorily validated in terms of linearity (r≥0.99), repeatability (RSD ≤ 8.4 %), and spike recovery (≥80 %). This method could be a promising approach for quality assessment of polysaccharides in herbal formulae.
KeywordDanggui Buxue Tang LC–MS Oligosaccharide Polysaccharide Qualitative and quantitative analysis
DOI10.1016/j.jpba.2020.113235
URLView the original
Language英语
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Cited Times [WOS]:1   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.School of Chinese Medicine,Hong Kong Baptist University,Hong Kong,Hong Kong
2.School of Pharmacy,Xinxiang Medical University,Xinxiang,China
3.Department of Chemistry,Hong Kong Baptist University,Hong Kong,Kowloon Tong,Hong Kong
4.State Key Laboratory of Quality Research in Chinese Medicine,Institute of Chinese Medical Sciences,University of Macau,Macao,Macao
Recommended Citation
GB/T 7714
Li,Li Feng,Wong,Tin Long,Zhang,Ji Xia,et al. An oligosaccharide-marker approach to quantify specific polysaccharides in herbal formula by LC-qTOF-MS: Danggui Buxue Tang, a case study[J]. Journal of Pharmaceutical and Biomedical Analysis,2020,185.
APA Li,Li Feng,Wong,Tin Long,Zhang,Ji Xia,Zhou,Li Shuang,Bai,Su Ping,Fung,Hau Yee,Cheng,Hui Yuan,Zhang,Quan Wei,Zheng,Hong Ming,Bao,Wan Rong,Ma,Dik Lung,Leung,Chung Hang,Zhang,Ge,Bian,Zhao Xiang,Lyu,Ai Ping,&Han,Quan Bin.(2020).An oligosaccharide-marker approach to quantify specific polysaccharides in herbal formula by LC-qTOF-MS: Danggui Buxue Tang, a case study.Journal of Pharmaceutical and Biomedical Analysis,185.
MLA Li,Li Feng,et al."An oligosaccharide-marker approach to quantify specific polysaccharides in herbal formula by LC-qTOF-MS: Danggui Buxue Tang, a case study".Journal of Pharmaceutical and Biomedical Analysis 185(2020).
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