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A new fluorescent technique for pesticide detection by using metal coordination polymer and nanozyme
Wei,Jinchao1,2; Xue,Yan2; Dong,Jiayi3; Wang,Shuangpeng3; Hu,Hao2; Gao,Hao1; Li,Peng2; Wang,Yitao2
2020-03-10
Source PublicationChinese Medicine (United Kingdom)
Volume15Issue:1
Abstract

Background: Chinese herbs have been widely used for thousands of years. In order to kill or control insects and fungus during the cultivation of herb plants, pesticides have played a significant role. More than 30 kinds of pesticides have been documented in the latest version of Chinese Pharmacopoeia. It is urgent to develop new analytical methods for pesticide detection. Methods: A fluorescent detection system was established by using Cerium based fluorescent polymer and Sm-CeO. As a new doped nanozyme, Sm-CeO exhibits OPH-like activity to hydrolyze OPP pesticide. Results: The morphology of the prepared CFP and Sm-CeO were characterized. The optimal conditions for CFP synthesis are CeCl (16 mmol L, 200 μL), ATP (4 mmol L, 200 μL) and Tris buffer (5 mmol L, 500 μL, pH 8.0). Sm-CeO shows the best mimic activity to hydrolyze OPP pesticide at pH = 8.0. The results exhibit good linear relationship between fluorescent quenching effect and MP in the range of 2-50 μmol L. Finally, this fluorescent technique was successfully applied in Poria cocos and Semen Coicis sample. Conclusions: It is the first report on OPP detection by using CFP and doped nanozyme. The successful application in real sample indicates this method is a rapid, reliable strategy to detect OPP in Chinese herbs.

KeywordAtp Cerium Oxide Fluorescent Methyl-paraoxon Nanozyme Organophosphorus Organophosphorus Hydrolase Para-nitrophenol Pesticide Polymer
DOI10.1186/s13020-020-00304-2
URLView the original
Indexed BySCIE
WOS Research AreaIntegrative & Complementary Medicine ; Pharmacology & Pharmacy
WOS SubjectIntegrative & Complementary Medicine ; Pharmacology & Pharmacy
WOS IDWOS:000521103000001
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Cited Times [WOS]:1   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorGao,Hao
Affiliation1.Institute of Traditional Chinese Medicine and Natural Products,College of Pharmacy,Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research,Jinan University,Guangzhou,China
2.State Key Laboratory of Quality Research in Chinese Medicine,Institute of Chinese Medical Sciences,University of Macau,Macao
3.Institute of Applied Physics and Materials Engineering,University of Macau,Macao
First Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Wei,Jinchao,Xue,Yan,Dong,Jiayi,et al. A new fluorescent technique for pesticide detection by using metal coordination polymer and nanozyme[J]. Chinese Medicine (United Kingdom),2020,15(1).
APA Wei,Jinchao,Xue,Yan,Dong,Jiayi,Wang,Shuangpeng,Hu,Hao,Gao,Hao,Li,Peng,&Wang,Yitao.(2020).A new fluorescent technique for pesticide detection by using metal coordination polymer and nanozyme.Chinese Medicine (United Kingdom),15(1).
MLA Wei,Jinchao,et al."A new fluorescent technique for pesticide detection by using metal coordination polymer and nanozyme".Chinese Medicine (United Kingdom) 15.1(2020).
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