UM
Hierarchical identification of bioactive components in a medicinal herb by preparative high-performance liquid chromatography and selective knock-out strategy
Qu,Cheng1; Wang,Lin Yan1; Lin,Hang1; Shang,Er Xin1; Tang,Yu Ping1; Yue,Shi Jun1; Jin,Yi1; Tao,Wei Wei1; Li,Shao Ping2; Hua,Yong Qing1; Liu,Pei1; Su,Shu Lan1; Zhou,Huiping3; Qian,Da Wei1; Duan,Jin Ao1
2017-02-20
Source PublicationJournal of Pharmaceutical and Biomedical Analysis
ISSN0731-7085
Volume135Pages:206-216
AbstractA novel and generally applicable approach was established to hierarchically identify the bioactive components of a medicinal herb by preparative high-performance liquid chromatography (prep-HPLC) and a selective knock-out strategy. In this study, the targeted components of an herbal medicine were separated and knocked out using prep-HPLC. Subsequently, the contributions of the different target components to the overall effect of the medicinal herb were comparatively evaluated and differentiated by a heat map and a 3D score plot. This approach was successfully applied to investigate the bioactive constituents of safflower. The contributions of 11 components to the overall effect of safflower were as follows: anhydrosafflor yellow B (10) > 6-hydroxykaempferol 3,6-di-O-β-D-glucoside (8) > hydroxysafflor yellow A (3) > kaempferol 3-O-β-rutinoside (11) > 6-hydroxykaempferol 3-O-β-rutinoside (9) > 6-hydroxykaempferol 3,6-di-O-β-D-glucoside-7-O-β-D-glucuronide (4) > 6-hydroxyapigenin 6-O-β-D-glucoside-7-O-β-D-glucuronide (6) > cytidine (1) > 6-hydroxykaempferol 3-O-β-rutinoside-6-O-β-D-glucoside (7) > 6-hydroxykaempferol 3,6,7-tri-O-β-D-glucoside (5) > adenosine (2). These results demonstrate that quinochalcone C-glycosides (3 and 10) and some flavonoid glycosides containing C7-OH (such as 8, 9 and 11) made a greater contribution to the overall effect of safflower than the other components that were knocked out. The results provided an important reference for improving quality control and further development of safflower products. And this approach should also be useful for investigating the bioactive constituents of other medicinal herbs.
KeywordComponent Contribution Herb High-performance liquid chromatography Knock-out Safflower
DOI10.1016/j.jpba.2016.12.027
URLView the original
Language英語English
Scopus ID2-s2.0-85007465182
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Cited Times [WOS]:9   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,Jiangsu Key Laboratory for High Technology Research of TCM Formulae,National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine,Nanjing University of Chinese Medicine,Nanjing,210023,China
2.State Key Laboratory of Quality Research in Chinse Medicine,University of Macau,Macao
3.Department of Microbiology and Immunology,Virginia Commonwealth University,Richmond,23298,United States
Recommended Citation
GB/T 7714
Qu,Cheng,Wang,Lin Yan,Lin,Hang,et al. Hierarchical identification of bioactive components in a medicinal herb by preparative high-performance liquid chromatography and selective knock-out strategy[J]. Journal of Pharmaceutical and Biomedical Analysis,2017,135:206-216.
APA Qu,Cheng,Wang,Lin Yan,Lin,Hang,Shang,Er Xin,Tang,Yu Ping,Yue,Shi Jun,Jin,Yi,Tao,Wei Wei,Li,Shao Ping,Hua,Yong Qing,Liu,Pei,Su,Shu Lan,Zhou,Huiping,Qian,Da Wei,&Duan,Jin Ao.(2017).Hierarchical identification of bioactive components in a medicinal herb by preparative high-performance liquid chromatography and selective knock-out strategy.Journal of Pharmaceutical and Biomedical Analysis,135,206-216.
MLA Qu,Cheng,et al."Hierarchical identification of bioactive components in a medicinal herb by preparative high-performance liquid chromatography and selective knock-out strategy".Journal of Pharmaceutical and Biomedical Analysis 135(2017):206-216.
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