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Synergistic immunomodulatory effect of complex polysaccharides from seven herbs and their major active fractions
Deng,Yong1; Xie,Jing1; Luo,Zhen2; Li,Shao Ping1; Zhao,Jing1
2020-12-15
Source PublicationInternational Journal of Biological Macromolecules
ISSN0141-8130
Volume165Pages:530-541
AbstractIn this report, we present the strategy for the revelation of synergistic effect and elucidation of active fractions from an immunomodulatory complex polysaccharide derived from seven herbs (Lentinula edodes, Ganodorma lucidum, Tremella fuciformis, Chrysanthemum, Lycium barbarum, Codonopsis pilosula and Poria cocos), a formula used as health product in China market, using the combination of HPSEC-MALLS, immunological bioassay and saccharide mapping analysis. The effects of complex polysaccharide and their fractions on RAW 246.7 macrophages demonstrated that the fractions (CD1, CD2, CD3) with molecular weight above 10 kDa exhibited immune activity by directly stimulated NO release and phagocytosis, and induced macrophages to secrete cytokines. Especially, fraction CD2 with molecular weight of 100–1000 kDa showed the strongest bioactivity (EC = 0.19 μg/mL) compared with their individual corresponding herbal polysaccharides fractions due to synergistic effect, which supported the scientific use of Chinese herbal mixture. Moreover, their chemical characters were analyzed by HPSEC-MALLS and saccharide mapping, and the original herbs, including L. edodes, G. lucidum, T. fuciformis and Chrysanthemum, responsible for the immunomodulatory activity were tentatively revealed. Results are beneficial for the quality analysis and formula optimization of complex polysaccharides in both biomedical and functional food field.
KeywordComplex polysaccharides Herbs Immunomodulatory activity Saccharide mapping Synergistic effect
DOI10.1016/j.ijbiomac.2020.09.199
URLView the original
Language英语
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Cited Times [WOS]:1   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.State Key Laboratory of Quality Research in Chinese Medicine,Institute of Chinese Medical Sciences,University of Macau,Macao,Macao
2.Infinitus (China) Co. Ltd.,Guangzhou,China
First Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Deng,Yong,Xie,Jing,Luo,Zhen,et al. Synergistic immunomodulatory effect of complex polysaccharides from seven herbs and their major active fractions[J]. International Journal of Biological Macromolecules,2020,165:530-541.
APA Deng,Yong,Xie,Jing,Luo,Zhen,Li,Shao Ping,&Zhao,Jing.(2020).Synergistic immunomodulatory effect of complex polysaccharides from seven herbs and their major active fractions.International Journal of Biological Macromolecules,165,530-541.
MLA Deng,Yong,et al."Synergistic immunomodulatory effect of complex polysaccharides from seven herbs and their major active fractions".International Journal of Biological Macromolecules 165(2020):530-541.
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