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Evaluation of amylase immobilization on nanoclay by size exclusion chromatography and its application for starch removal from plant extracts of herbal medicines
Shakerian,Farid; Zhao,Jing; Li,Shao Ping
2021-02-01
Source PublicationProcess Biochemistry
ISSN1359-5113
Volume101Pages:36-41
AbstractA simple method for immobilization of amylase (Am) on montmorillonite (Mt) was developed. Direct immobilization of crude commercial Am was initially evaluated by size exclusion chromatography by considering Am peaks (in comparison to the pure enzyme from the same source), while the immobilized enzyme performance was monitored by anion exchange chromatography. The immobilized Am showed approximately 55.6 ± 0.7 % (n = 3) of the activity of the free enzyme. Both free and immobilized Am showed maximum performance in neutral pH (pH ∼ 7). The immobilized Am maintained more than 60 % of its initial activity after 10 reuse cycles. The immobilized enzyme was successfully applied for starch removal in crude carbohydrates extracted from medicinal plant samples.
KeywordAmylase immobilization Medicinal plants Montmorillonite Starch removal
DOI10.1016/j.procbio.2020.11.003
URLView the original
Language英语
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Document TypeJournal article
CollectionUniversity of Macau
Corresponding AuthorZhao,Jing
AffiliationState Key Laboratory of Quality Research in Chinese Medicine,Institute of Chinese Medical Sciences,University of Macau,Macau,China
First Author AffilicationInstitute of Chinese Medical Sciences
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Shakerian,Farid,Zhao,Jing,Li,Shao Ping. Evaluation of amylase immobilization on nanoclay by size exclusion chromatography and its application for starch removal from plant extracts of herbal medicines[J]. Process Biochemistry,2021,101:36-41.
APA Shakerian,Farid,Zhao,Jing,&Li,Shao Ping.(2021).Evaluation of amylase immobilization on nanoclay by size exclusion chromatography and its application for starch removal from plant extracts of herbal medicines.Process Biochemistry,101,36-41.
MLA Shakerian,Farid,et al."Evaluation of amylase immobilization on nanoclay by size exclusion chromatography and its application for starch removal from plant extracts of herbal medicines".Process Biochemistry 101(2021):36-41.
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