UM
Tetramethylpyrazine and Paeoniflorin Inhibit Oxidized LDL-Induced Angiogenesis in Human Umbilical Vein Endothelial Cells via VEGF and Notch Pathways
Yuan,Rong1,2; Shi,Weili1; Xin,Qiqi1; Yang,Binrui3; Hoi,Maggie Puiman3; Lee,Simon Mingyuan3; Cong,Weihong1; Chen,Keji1
2018
Source PublicationEvidence-based Complementary and Alternative Medicine
ISSN1741-427X
Volume2018
AbstractAtherosclerotic plaque angiogenesis is key factor in plaque instability and vulnerability, and low concentrations of oxidized low density lipoprotein (ox-LDL) promote the in vitro angiogenesis of endothelial cells and play an important role in plaque angiogenesis. Ligusticum chuanxiong Hort. and Radix Paeoniae Rubra herb pair in Chinese medicine obtains the optimum therapeutic efficacy in atherosclerosis, and their major active ingredients tetramethylpyrazine (TMP) and paeoniflorin (PF) are reported to alleviate atherosclerosis. The aim of this study was to investigate the effects of TMP and PF on ox-LDL-induced angiogenesis and the underlying mechanism. Human umbilical vein endothelial cells (HUVECs) were incubated with ox-LDL and were then treated with TMP, PF, or a combination of TMP and PF. Cell proliferation, migration, tube formation, and the expression of angiogenesis-related proteins were measured. Synergism was evaluated using the combination index in cell proliferation. We found that TMP and PF attenuated the in vitro angiogenesis in ox-LDL-induced HUVECs. In addition, the combination of TMP and PF not only inhibited the ox-LDL-induced expression of CD31, vascular endothelial growth factor (VEGF), and VEGF receptor 2 (VEGFR2) but also decreased the ox-LDL-induced expression of Notch1, Jagged1, and Hes1. In summary, the combination of TMP and PF suppresses ox-LDL-induced angiogenesis in HUVECs by inhibiting both the VEGF/VEGFR2 and the Jagged1/Notch1 signaling pathways, which might contribute to the stability of plaques in atherosclerosis.
DOI10.1155/2018/3082507
URLView the original
Language英语
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Cited Times [WOS]:3   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Corresponding AuthorCong,Weihong
Affiliation1.Laboratory of Cardiovascular Diseases,Xiyuan Hospital,China Academy of Chinese Medical Sciences,Beijing,100091,China
2.Graduate School,Beijing University of Chinese Medicine,Beijing,100029,China
3.State Key Laboratory of Quality Research in Chinese Medicine,Institute of Chinese Medical Sciences,University of Macau,Macao
Recommended Citation
GB/T 7714
Yuan,Rong,Shi,Weili,Xin,Qiqi,et al. Tetramethylpyrazine and Paeoniflorin Inhibit Oxidized LDL-Induced Angiogenesis in Human Umbilical Vein Endothelial Cells via VEGF and Notch Pathways[J]. Evidence-based Complementary and Alternative Medicine,2018,2018.
APA Yuan,Rong.,Shi,Weili.,Xin,Qiqi.,Yang,Binrui.,Hoi,Maggie Puiman.,...&Chen,Keji.(2018).Tetramethylpyrazine and Paeoniflorin Inhibit Oxidized LDL-Induced Angiogenesis in Human Umbilical Vein Endothelial Cells via VEGF and Notch Pathways.Evidence-based Complementary and Alternative Medicine,2018.
MLA Yuan,Rong,et al."Tetramethylpyrazine and Paeoniflorin Inhibit Oxidized LDL-Induced Angiogenesis in Human Umbilical Vein Endothelial Cells via VEGF and Notch Pathways".Evidence-based Complementary and Alternative Medicine 2018(2018).
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