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Deciphering the microbial taxonomy and functionality of two diverse mangrove ecosystems and their potential abilities to produce bioactive compounds
Liao,Shuilin1,2; Wang,Yayu2,7; Liu,Huan2; Fan,Guangyi3,4; Sahu,Sunil Kumar2; Jin,Tao3,4; Chen,Jianwei3,4; Zhang,Pengfan1,2; Gram,Lone7; Strube,Mikael Lenz7; Shi,Qiong1,2; Lee,Simon Ming Yuen5,6; Liu,Xin2,4,8
2020-10-27
Source PublicationmSystems
Volume5Issue:5
AbstractMangroves, as important and special ecosystems, create unique ecological environments for examining the microbial gene capacity and potential for producing bioactive compounds. However, little is known about the biogeochemical implications of microbiomes in mangrove ecosystems, especially the variations between pristine and anthropogenic mangroves. To elucidate this, we investigated the microbial taxonomic and functional shifts of the mangrove microbiomes and their potential for bioactive compounds in two different coastal mangrove ecosystems in southern China. A gene catalogue, including 87 million unique genes, was constructed, based on deep shotgun metagenomic sequencing. Differentially enriched bacterial and archaeal taxa between pristine mangroves (Guangxi) and anthropogenic mangroves (Shenzhen) were found. The Nitrospira and ammonia-oxidizing archaea, specifically, were more abundant in Shenzhen mangroves, while sulfate-reducing bacteria and methanogens were more abundant in Guangxi mangroves. The results of functional analysis were consistent with the taxonomic results, indicating that the Shenzhen mangrove microbiome has a higher abundance of genes involved in nitrogen metabolism while the Guangxi mangrove microbiome has a higher capacity for sulfur metabolism and methanogenesis. Biosynthetic gene clusters were identified in the metagenome data and in hundreds of de novo reconstructed nonredundant microbial genomes, respectively. Notably, we found different biosynthetic potential in different taxa, and we identified three high quality and novel Acidobacteria genomes with a large number of BGCs. In total, 67,278 unique genes were annotated with antibiotic resistance, indicating the prevalence and persistence in multidrug-resistant genes in the mangrove microbiome. IMPORTANCE This study comprehensively described the taxonomy and functionality of mangrove microbiomes, including their capacity for secondary metabolite biosynthesis and their ability to resist antibiotics. The microbial taxonomic and functional characteristics differed between geographical locations, corresponding to the environmental condition of two diverse mangrove regions. A large number of microbial biosynthetic gene clusters encoding novel bioactivities were found, and this can serve as a valuable resource to guide novel bioactive compound discovery for potential clinical uses.
KeywordAntibiotic resistance genes Biosynthesis gene clusters Mangroves Metagenomics Rhizosphere
DOI10.1128/mSystems.00851-19
URLView the original
Language英语
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Document TypeJournal article
CollectionUniversity of Macau
Corresponding AuthorLiu,Xin
Affiliation1.BGI Education Center,University of Chinese Academy of Sciences,Shenzhen,China
2.State Key Laboratory of Agricultural Genomics,BGI-Shenzhen,Shenzhen,China
3.BGI-Qingdao,Qingdao,China
4.BGI-Shenzhen,Shenzhen,China
5.State Key Laboratory of Quality Research in Chinese Medicine,University of Macau,Macao
6.Institute of Chinese Medical Sciences,University of Macau,Macao
7.Department of Biotechnology and Biomedicine,Technical University of Denmark,Kongens Lyngby,Denmark
8.BGI-Fuyang,Fuyang,China
Recommended Citation
GB/T 7714
Liao,Shuilin,Wang,Yayu,Liu,Huan,et al. Deciphering the microbial taxonomy and functionality of two diverse mangrove ecosystems and their potential abilities to produce bioactive compounds[J]. mSystems,2020,5(5).
APA Liao,Shuilin.,Wang,Yayu.,Liu,Huan.,Fan,Guangyi.,Sahu,Sunil Kumar.,...&Liu,Xin.(2020).Deciphering the microbial taxonomy and functionality of two diverse mangrove ecosystems and their potential abilities to produce bioactive compounds.mSystems,5(5).
MLA Liao,Shuilin,et al."Deciphering the microbial taxonomy and functionality of two diverse mangrove ecosystems and their potential abilities to produce bioactive compounds".mSystems 5.5(2020).
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