A digital microfluidic system with 3D microstructures for single-cell culture
Zhai,Jiao1; Li,Haoran1,2; Wong,Ada Hang Heng3; Dong,Cheng1; Yi,Shuhong4; Jia,Yanwei1,2,3; Mak,Pui In1,2; Deng,Chu Xia3; Martins,Rui P.1,2,5
Source PublicationMicrosystems and Nanoengineering

Despite the precise controllability of droplet samples in digital microfluidic (DMF) systems, their capability in isolating single cells for long-time culture is still limited: typically, only a few cells can be captured on an electrode. Although fabricating small-sized hydrophilic micropatches on an electrode aids single-cell capture, the actuation voltage for droplet transportation has to be significantly raised, resulting in a shorter lifetime for the DMF chip and a larger risk of damaging the cells. In this work, a DMF system with 3D microstructures engineered on-chip is proposed to form semi-closed micro-wells for efficient single-cell isolation and long-time culture. Our optimum results showed that approximately 20% of the micro-wells over a 30 × 30 array were occupied by isolated single cells. In addition, low-evaporation-temperature oil and surfactant aided the system in achieving a low droplet actuation voltage of 36V, which was 4 times lower than the typical 150 V, minimizing the potential damage to the cells in the droplets and to the DMF chip. To exemplify the technological advances, drug sensitivity tests were run in our DMF system to investigate the cell response of breast cancer cells (MDA-MB-231) and breast normal cells (MCF-10A) to a widely used chemotherapeutic drug, Cisplatin (Cis). The results on-chip were consistent with those screened in conventional 96-well plates. This novel, simple and robust single-cell trapping method has great potential in biological research at the single cell level.

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Indexed BySCIE
WOS Research AreaScience & Technology - Other Topics ; Instruments & Instrumentation
WOS SubjectNanoscience & Nanotechnology ; Instruments & Instrumentation
WOS IDWOS:000512731300002
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Cited Times [WOS]:9   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
Corresponding AuthorJia,Yanwei
Affiliation1.State-Key Laboratory of Analog and Mixed-Signal VLSI,Institute of Microelectronics,University of Macau,Macao
2.Faculty of Science and Technology-ECE,University of Macau,Macao
3.Cancer Center,Faculty of Health Sciences,University of Macau,Macao
4.Liver Transplantation Center,the Third Affiliated Hospital,Sun Yat-sen University,Guangzhou,510000,China
5.on leave from Instituto Superior Técnico,Universidade de Lisboa,Lisboa,Portugal
First Author AffilicationUniversity of Macau
Corresponding Author AffilicationUniversity of Macau;  Faculty of Science and Technology;  Cancer Centre
Recommended Citation
GB/T 7714
Zhai,Jiao,Li,Haoran,Wong,Ada Hang Heng,et al. A digital microfluidic system with 3D microstructures for single-cell culture[J]. Microsystems and Nanoengineering,2020,6(1).
APA Zhai,Jiao,Li,Haoran,Wong,Ada Hang Heng,Dong,Cheng,Yi,Shuhong,Jia,Yanwei,Mak,Pui In,Deng,Chu Xia,&Martins,Rui P..(2020).A digital microfluidic system with 3D microstructures for single-cell culture.Microsystems and Nanoengineering,6(1).
MLA Zhai,Jiao,et al."A digital microfluidic system with 3D microstructures for single-cell culture".Microsystems and Nanoengineering 6.1(2020).
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