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Hydrodynamic-flow-enhanced rapid mixer for isothermal DNA hybridization kinetics analysis on digital microfluidics platform
Mingzhong Li1,2; Cheng Dong1; Man-Kay Law1; Yanwei Jia1; Pui-In Mak1,2; Rui P.Martins1,2,3
AbstractDNA hybridization kinetics has been playing a critical role in molecular diagnostics for binding discrimination, but its study on digital microfluidic (DMF) systems is ultimately restrained by the laminar flow condition. The kinetic mixing technique is widely employed to ensure a fast reaction rate, but poses intrinsic risk in cross contamination and exhibits instable fluorescence intensity during the droplet transportation. While the electrothermal technique can provide stationary droplet mixing through the established thermal gradient within the hybridization solution, the significant increase in the droplet temperature will inevitably undermine the hybridization equilibrium and jeopardize the binding discrimination. To enhance the hybridization efficiency while ensuring a stable droplet temperature (within ±0.1℃), this paper presents a DMF platform that can perform isothermal hydrodynamic-flow-enhanced droplet mixing. Specifically, with a single electrode, droplet-boundary oscillation under a slow AC actuation is studied for improving the reaction rate. The dependencies between the mixing efficiency and the actuation voltage, actuation frequency and the spacer thickness are also systematically studied. Reliable mixing efficiency improvement is further validated over a wide range of solute concentrations. The results from real-time on-chip DNA hybridization kinetics with stationary droplets using the complete sandwiched DMF system shows that the proposed rapid mixer can achieve the same hybridization equilibrium with >13 times faster reaction rate when compared to the reference one through pure diffusion, while preventing biased hybridization kinetics as demonstrated in the electrothermal technique.
KeywordDna Hybridization Kinetics Digital Microfluidics Hydrodynamic Flow Isothermal Rapid Mixing
Indexed BySCIE
WOS Research AreaChemistry ; Electrochemistry ; Instruments & Instrumentation
WOS SubjectChemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation
WOS IDWOS:000461341700048
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Cited Times [WOS]:2   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Faculty of Science and Technology
Affiliation1.State Key Laboratory of Analog and Mixed-Signal VLSI, University of Macau, Macao, China
2.Faculty of Science and Technology – Electrical and Computer Engineering, University of Macau, Macao, China
3.On leave from Instituto Superior Técnico, Universidade de Lisboa, Lisboa, Portugal
First Author AffilicationUniversity of Macau;  Faculty of Science and Technology
Recommended Citation
GB/T 7714
Mingzhong Li,Cheng Dong,Man-Kay Law,et al. Hydrodynamic-flow-enhanced rapid mixer for isothermal DNA hybridization kinetics analysis on digital microfluidics platform[J]. SENSORS AND ACTUATORS B-CHEMICAL,2019,287:390-397.
APA Mingzhong Li,Cheng Dong,Man-Kay Law,Yanwei Jia,Pui-In Mak,&Rui P.Martins.(2019).Hydrodynamic-flow-enhanced rapid mixer for isothermal DNA hybridization kinetics analysis on digital microfluidics platform.SENSORS AND ACTUATORS B-CHEMICAL,287,390-397.
MLA Mingzhong Li,et al."Hydrodynamic-flow-enhanced rapid mixer for isothermal DNA hybridization kinetics analysis on digital microfluidics platform".SENSORS AND ACTUATORS B-CHEMICAL 287(2019):390-397.
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