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Formulation of sustained-release microspheres of granulocyte macrophage colony stimulating factor by freezing-induced phase separation with dextran and encapsulation with blended polymers
Xin-ran Zhang1; Ying Zheng2; Tuo Jin3; Albert H. L. Chow1
2011
Source PublicationJournal of Microencapsulation
ISSN0265-2048
Volume28Issue:8Pages:743-751
Abstract

This study aimed to assess the potential merits of formulating sustained-release microspheres of recombinant human granulocyte macrophage colony stimulating factor (rhGM-CSF) via freezing-induced phase separation (FIPS) of the protein with dextran followed by encapsulation with binary mixture of poly(lactic-co-glycolic acid) (PLGA) 2A (MW∼12K) and 3A (MW∼47K) or of PLGA2A and polylactic acid (PLA; MW∼83K). The formulated dextran particles and microspheres were characterized in vitro for loading, aggregation, bioactivity and release behavior of the protein where appropriate. rhGM-CSF retained about 60% of bioactivity with no significant aggregation after each formulation step. Encapsulation of protein-loaded dextran particles attained only 80% with the PLGA2A and PLGA3A blend, but 100% with the PLGA2A and PLA mixture. The former formulation exhibited a triphasic in-vitro release profile typical of PLGA microspheres while the latter revealed a much lower initial burst followed by a steady and complete release of rhGM-CSF with preserved bioactivity over a 15-day period.

KeywordFreezing-induced Phase Separation Dextran-stabilized Protein Plga And Pla Polymer Blends Rhgm-csf Microspheres
DOIhttps://doi.org/10.3109/02652048.2011.615950
Indexed BySCI
Language英语
WOS Research AreaChemistry ; Engineering ; Pharmacology & Pharmacy
WOS SubjectChemistry, Applied ; Engineering, Chemical ; Pharmacology & Pharmacy
WOS IDWOS:000297353300006
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Cited Times [WOS]:4   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorAlbert H. L. Chow
Affiliation1.School of Pharmacy, The Chinese University of Hong Kong, Hong Kong, China
2.Institute of Chinese Medical Sciences, University of Macau, Macau, China
3.School of Pharmacy, Shanghai Jiao Tong University, Shanghai, China
Recommended Citation
GB/T 7714
Xin-ran Zhang,Ying Zheng,Tuo Jin,et al. Formulation of sustained-release microspheres of granulocyte macrophage colony stimulating factor by freezing-induced phase separation with dextran and encapsulation with blended polymers[J]. Journal of Microencapsulation,2011,28(8):743-751.
APA Xin-ran Zhang,Ying Zheng,Tuo Jin,&Albert H. L. Chow.(2011).Formulation of sustained-release microspheres of granulocyte macrophage colony stimulating factor by freezing-induced phase separation with dextran and encapsulation with blended polymers.Journal of Microencapsulation,28(8),743-751.
MLA Xin-ran Zhang,et al."Formulation of sustained-release microspheres of granulocyte macrophage colony stimulating factor by freezing-induced phase separation with dextran and encapsulation with blended polymers".Journal of Microencapsulation 28.8(2011):743-751.
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