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Analysis of Cordycepsby multi-column liquid chromatography
Qian, Zhengming1,2; Li, Shaoping1

Cordyceps is a famous traditional Chinese medicine (TCM) that has been used in China for hundreds of years. In the present study a multi-column liquid chromatography (MC-LC) system was developed for the qualitative analysis of macromolecules and micromolecules in Cordyceps. The MC-LC system includes a size exclusion pre-column, a size exclusion column (SEC) and a reversed phase column (RP) which were controlled by column-switching valves. The sample was separated by the size exclusion pre-column into two fractions (macromolecules and micromolecules). These fractions were further separated on SEC and RP columns, respectively. A diode array detector (DAD) and a mass spectrometer (MS) were used to detect the components. This MC-LC method was utilized for analysis of Cordyceps samples. Two macromolecular peaks and 15 micromolecular peaks were found in Cordyceps, and 11 of the micromolecular peaks were identified as adenosine-5'-monophosphate (AMP), phenylalanine, uridine, hypoxanthine, inosine, guanine, guanosine, deoxyadenosine-5'-monophosphate (dAMP), adenosine, adenine and cordycepin (or its isomer). This method is useful for quality control of Cordyceps. (C) 2017 Chinese Pharmaceutical Association and Institute of Materia Medica, Chinese Academy of Medical Sciences. Production and hosting by Elsevier B.V.

KeywordCordycepie Macromocules Micromolecules Multi-column Size Exclusion Column
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Indexed BySCIE
WOS Research AreaPharmacology & Pharmacy
WOS SubjectPharmacology & Pharmacy
WOS IDWOS:000397060100012
The Source to ArticleWOS
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Cited Times [WOS]:6   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorLi, Shaoping
Affiliation1.Institute of Chinese Medical Sciences, University of Macau, Macao, China
2.Key Laboratory of State Administration of Traditional Chinese Medicine, Sunshine Lake Pharma Co., Ltd., Dongguan 523850, China
First Author AffilicationInstitute of Chinese Medical Sciences
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Qian, Zhengming,Li, Shaoping. Analysis of Cordycepsby multi-column liquid chromatography[J]. ACTA PHARMACEUTICA SINICA B,2017,7(2):202-207.
APA Qian, Zhengming,&Li, Shaoping.(2017).Analysis of Cordycepsby multi-column liquid chromatography.ACTA PHARMACEUTICA SINICA B,7(2),202-207.
MLA Qian, Zhengming,et al."Analysis of Cordycepsby multi-column liquid chromatography".ACTA PHARMACEUTICA SINICA B 7.2(2017):202-207.
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