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Cytotoxic and apoptosis-inducing activity of C-21 steroids from the roots of Cynanchum atratum
Zhang, Jian1,2; Ma, Lin1,3; Wu, Zheng-Feng1,3; Yu, Shu-Le1,3; Wang, Lei4; Ye, Wen-Cai4; Zhang, Qing-Wen5; Yin, Zhi-Qi3
2017-06
Source PublicationSTEROIDS
ISSN0039-128X
Volume122Pages:1-8
Abstract

Two new (1-2) and two known C-21 steroids (3-4) were isolated from the roots of Cynanchum atratum. Their structures were elucidated by detailed 1D and 2D spectroscopic. The MIT assay showed that compounds 1-4 displayed obvious cytotoxic activities against HepG2 cells with IC50 values ranging from 10.19 mu M to 76.12 mu M. Compounds 1-3 also exhibited cytotoxic effects in A549 cells with IC50 values of 30.87-95.39 mu M. Compound 3 showed the antiproliferative activity via GO/G1 cell cycle arrest and proapoptosis in HepG2 cells by Flowcytometry analysis. Western blotting analysis revealed that compound 3 could induce HepG2 cell apoptosis via the mitochondrial pathway by downregulating Bcl-2 expression, upregulating Bax protein expression, and activating caspase-9 and caspase-3. (C) 2017 Elsevier Inc. All rights reserved.

KeywordCynanchum Atratum C-21 Steroids Cytotoxic Activity Apoptosis Mitochondrial Pathway
DOI10.1016/j.steroids.2017.03.004
URLView the original
Indexed BySCIE ; IC
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Endocrinology & Metabolism
WOS SubjectBiochemistry & Molecular Biology ; Endocrinology & Metabolism
WOS IDWOS:000401387200001
PublisherELSEVIER SCIENCE INC
The Source to ArticleWOS
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Cited Times [WOS]:11   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorYin, Zhi-Qi
Affiliation1.Laboratory of Translational Medicine, Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing, PR China
2.Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, PR China
3.Department of Natural Medicinal Chemistry & State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, PR China
4.Institute of Traditional Chinese Medicine and Natural Products & Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine and New Drugs Research, Jinan University, Guangzhou, PR China
5.Institute of Chinese Medical Sciences (ICMS), University of Macau, PR China
Recommended Citation
GB/T 7714
Zhang, Jian,Ma, Lin,Wu, Zheng-Feng,et al. Cytotoxic and apoptosis-inducing activity of C-21 steroids from the roots of Cynanchum atratum[J]. STEROIDS,2017,122:1-8.
APA Zhang, Jian,Ma, Lin,Wu, Zheng-Feng,Yu, Shu-Le,Wang, Lei,Ye, Wen-Cai,Zhang, Qing-Wen,&Yin, Zhi-Qi.(2017).Cytotoxic and apoptosis-inducing activity of C-21 steroids from the roots of Cynanchum atratum.STEROIDS,122,1-8.
MLA Zhang, Jian,et al."Cytotoxic and apoptosis-inducing activity of C-21 steroids from the roots of Cynanchum atratum".STEROIDS 122(2017):1-8.
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