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Chikusetsusaponin IVa methyl ester induces G1 cell cycle arrest, triggers apoptosis and inhibits migration and invasion in ovarian cancer cells
Chen, Xin1; Wu, Qiu-Shuang1; Meng, Fan-Cheng1; Tang, Zheng-Hai1; Chen, Xiuping1; Lin, Li-Gen1; Chen, Ping2; Qiang, Wen-An3; Wang, Yi-Tao1; Zhang, Qing-Wen1; Lu, Jin-Jian1
2017-06-15
Source PublicationPHYTOMEDICINE
ISSN0944-7113
Volume29Pages:E1-E1
Abstract

Background: Panacis Japonici Rhizoma (PJR) is one of the most famous Chinese medical herbs that is known for exhibiting potential anti-cancer effects. Purpose: This study aims to isolate and investigate the anti-cancer potential of saponins from PJR in ovarian cancer cells.

Methods: The compounds were separated by comprehensive chromatographic methods. By comparison of the 1H- and 13C NMR data, as well as the HR-ESI-MS data, with the corresponding references, the structures of compounds were determined. MTT assay was performed to evaluate cell viability, along with flow cytometry for cell cycle analysis. JC-1 staining, Annexin V-PI double staining as well as Hoechst 33; 342 staining were used for detecting cell apoptosis. Western blot analysis was conducted to determine the relative protein level. Transwell assays were performed to investigate the effect of the saponin on cell migration and invasion and zymography experiments were used to detect the enzymatic activities.

Results: Eleven saponins were isolated from PJR and their anti-proliferative effects were evaluated in hu- man ovarian cancer cells. Chikusetsusaponin IVa methyl ester (1) exhibited the highest anti-proliferative potential among these isolates with the IC50 values at less than 10 μM in both ovarian cancer A2780 and HEY cell lines. Compound 1 induced G1 cell cycle arrest accompanied with an S phase decrease, and down-regulated the expression of cyclin D1, CDK2, and CDK6. Further study showed that compound 1 ef- fectively decreased the cell mitochondrial membrane potential, increased the annexin V positive cells and nuclear chromatin condensation, as well as enhanced the expression of cleaved PARP, Bax and cleaved- caspase 3 while decreasing that of Bcl-2. Moreover, compound 1 suppressed the migration and invasion of HEY and A2780 cells, down-regulated the expression of Cdc42, Rac, RohA, MMP2 and MMP9, and de- creased the enzymatic activities of MMP2 and MMP9.

Conclusion: These results provide a comprehensive evaluation of compound 1 as a potential agent for the treatment of ovarian cancer. 

DOI10.1016/j.phymed.2017.05.003
URLView the original
Indexed BySCIE
Language英語English
WOS Research AreaPlant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
WOS SubjectPlant Sciences ; Chemistry, Medicinal ; Integrative & Complementary Medicine ; Pharmacology & Pharmacy
WOS IDWOS:000388090000002
PublisherELSEVIER GMBH, URBAN & FISCHER VERLAG
The Source to ArticleWOS
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Cited Times [WOS]:21   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Corresponding AuthorZhang, Qing-Wen; Lu, Jin-Jian
Affiliation1.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China
2.College of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan430023, China
3.Department of Obstetrics and Gynecology-Division of Reproductive Science in Medicine , Department of Pathology, Robert H. Lurie Comprehensive Cancer Center, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA
First Author AffilicationInstitute of Chinese Medical Sciences
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Chen, Xin,Wu, Qiu-Shuang,Meng, Fan-Cheng,et al. Chikusetsusaponin IVa methyl ester induces G1 cell cycle arrest, triggers apoptosis and inhibits migration and invasion in ovarian cancer cells[J]. PHYTOMEDICINE,2017,29:E1-E1.
APA Chen, Xin,Wu, Qiu-Shuang,Meng, Fan-Cheng,Tang, Zheng-Hai,Chen, Xiuping,Lin, Li-Gen,Chen, Ping,Qiang, Wen-An,Wang, Yi-Tao,Zhang, Qing-Wen,&Lu, Jin-Jian.(2017).Chikusetsusaponin IVa methyl ester induces G1 cell cycle arrest, triggers apoptosis and inhibits migration and invasion in ovarian cancer cells.PHYTOMEDICINE,29,E1-E1.
MLA Chen, Xin,et al."Chikusetsusaponin IVa methyl ester induces G1 cell cycle arrest, triggers apoptosis and inhibits migration and invasion in ovarian cancer cells".PHYTOMEDICINE 29(2017):E1-E1.
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