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Enhanced Phototherapy by Nanoparticle-Enzyme via Generation and Photolysis of Hydrogen Peroxide
Chang, Kaiwen1,2; Liu, Zhihe3; Fang, Xiaofeng3; Chen, Haobin3; Men, Xiaoju3; Yuan, Ye3; Sun, Kai3; Zhang, Xuanjun2; Yuan, Zhen2; Wu, Changfeng1
Source PublicationNANO LETTERS

Light has been widely used for cancer therapeutics such as photodynamic therapy (PDT) and photothermal therapy. This paper describes a strategy called enzyme-enhanced phototherapy (EEPT). for cancer treatment. We constructed a nanoparticle platform by covalent conjugation of glucose oxidase (GOx) to small polymer dots, which could be persistently immobilized into a tumor. While the malignant tumors have high glucose uptake, the GOx efficiently catalyzes the glucose oxidation with simultaneous generation of H2O2. Under light irradiation, the in situ generated H2O2 was photolyzed to produce hydroxyl radical, the most reactive oxygen species, for killing cancer cells. In vitro assays indicated that the cancer cells were destroyed by using a nanoparticle concentration at 0.2 mu g/mL and a light dose of similar to 120 J/cm(2), indicating the significantly enhanced efficiency of the EEPT method when compared to typical PDT that requires a photosensitizer of >10 mu g/mL for effective cell killing under the same light dose. Furthermore, remarkable inhibition of tumor growth was observed in xenograft-bearing mice, indicating the promise of the EEPT approach for cancer therapeutics.

KeywordPolymer Dot Glucose Oxidase Hydrogen Peroxide Hydroxyl Radical Enzyme-enhanced Phototherapy
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Indexed BySCI
WOS Research AreaChemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
WOS SubjectChemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied ; Physics, Condensed Matter
WOS IDWOS:000405643300047
The Source to ArticleWOS
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Cited Times [WOS]:108   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Affiliation1.Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, Guangdong 518055, China
2.Faculty of Health Sciences, University of Macau, Taipa, Macau SAR China
3.State Key Laboratory on Integrated Optoelectronics, College of Electronic Science and Engineering, Jilin University, Changchun, Jilin 130012, China
First Author AffilicationFaculty of Health Sciences
Recommended Citation
GB/T 7714
Chang, Kaiwen,Liu, Zhihe,Fang, Xiaofeng,et al. Enhanced Phototherapy by Nanoparticle-Enzyme via Generation and Photolysis of Hydrogen Peroxide[J]. NANO LETTERS,2017,17(7):4323-4329.
APA Chang, Kaiwen.,Liu, Zhihe.,Fang, Xiaofeng.,Chen, Haobin.,Men, Xiaoju.,...&Wu, Changfeng.(2017).Enhanced Phototherapy by Nanoparticle-Enzyme via Generation and Photolysis of Hydrogen Peroxide.NANO LETTERS,17(7),4323-4329.
MLA Chang, Kaiwen,et al."Enhanced Phototherapy by Nanoparticle-Enzyme via Generation and Photolysis of Hydrogen Peroxide".NANO LETTERS 17.7(2017):4323-4329.
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