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Rhizosphere competitiveness of trichloroethylene-degrading, poplar-colonizing recombinant bacteria
Shim H.; Chauhan S.; Ryoo D.; Bowers K.; Thomas S.M.; Canada K.A.; Burken J.G.; Wood T.K.
2000-11-18
Source PublicationApplied and Environmental Microbiology
ISSN00992240
Volume66Issue:11Pages:4673-4678
AbstractIndigenous bacteria from poplar tree (Populus canadensis var. eugenei 'Imperial Carolina') and southern California shrub rhizospheres, as well as two tree-colonizing Rhizobium strains (ATCC 10320 and ATCC 35645), were engineered to express constitutively and stably toluene o-monooxygenase (TOM) from Burkholderia cepacia G4 by integrating the tom locus into the chromosome. The poplar and Rhizobium recombinant bacteria degraded trichloroethylene at a rate of 0.8 to 2.1 nmol/min/mg of protein and were competitive against the unengineered hosts in wheat and barley rhizospheres for 1 month (colonization occurred at a level of 1.0 x 10 to 23 x 10 CFU/cm of root). In addition, six of these recombinants colonized poplar roots stably and competitively with populations as large as 79% ± 12% of all rhizosphere bacteria after 28 days (0.2 x 10 to 31 x 10 CFU/cm of root). Furthermore, five of the most competitive poplar recombinants (e.g., Pb3-1 and Pb5-1, which were identified as Pseudomonas sp. strain PsK recombinants) retained the ability to express TOM for 29 days as 100% ± 0% of the recombinants detected in the poplar rhizosphere expressed TOM constitutively.
DOI10.1128/AEM.66.11.4673-4678.2000
URLView the original
Language英語
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Cited Times [WOS]:52   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
AffiliationUniversity of Connecticut
Recommended Citation
GB/T 7714
Shim H.,Chauhan S.,Ryoo D.,et al. Rhizosphere competitiveness of trichloroethylene-degrading, poplar-colonizing recombinant bacteria[J]. Applied and Environmental Microbiology,2000,66(11):4673-4678.
APA Shim H.,Chauhan S.,Ryoo D.,Bowers K.,Thomas S.M.,Canada K.A.,Burken J.G.,&Wood T.K..(2000).Rhizosphere competitiveness of trichloroethylene-degrading, poplar-colonizing recombinant bacteria.Applied and Environmental Microbiology,66(11),4673-4678.
MLA Shim H.,et al."Rhizosphere competitiveness of trichloroethylene-degrading, poplar-colonizing recombinant bacteria".Applied and Environmental Microbiology 66.11(2000):4673-4678.
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