UM
Aerobic degradation of mixtures of tetrachloroethylene, trichloroethylene, dichloroethylenes, and vinyl chloride by toluene-o-xylene monooxygenase of Pseudomonas stutzeri OX1
Shim H.4; Ryoo D.4; Barbieri P.5; Wood T.K.4
2001
Source PublicationApplied Microbiology and Biotechnology
ISSN01757598
Volume56Issue:1-2Pages:265-269
AbstractA recombinant strain of Escherichia coli (JM109/pBZ1260) expressing constitutively toluene-o-xylene monooxygenase (ToMO) of Pseudomonas stutzeri OX1 degraded binary mixtures (100 μM each) of tetrachloroethylene (PCE) with either trichloroethylene (TCE), 1,1-dichloroethylene (1,1-DCE), cis-dichloroethylene (cis-DCE), trans-1,2-dichloroethylene (trans-DCE), or vinyl chloride (VC). PCE degradation was 8-20% for these binary mixtures, while TCE and trans-DCE with PCE were degraded at 19%, 1,1-DCE at 37%, cis-DCE at 97%, and VC at 27%. The host P. stutzeri OX1 was also found to degrade binary mixtures of PCE/TCE, PCE/cis-DCE, and PCE/VC when induced with toluene. Degradation of quaternary mixtures of PCE/TCE/trans-DCE/VC and PCE/TCE/cis-DCE/VC by JM109/pBZ1260 were also investigated as well as mixtures of PCE/TCE/trans-DCE/1,1-DCE/cis-DCE/VC; when all the chlorinated compounds were present, the best degradation occurred with 24-51% removal of each. For these degradation reactions, 39-85% of the stoichiometric chloride expected from complete degradation of the chlorinated ethenes was detected. The time course of PCE/TCE/1,1-DCE degradation was also measured for a mixture of 8, 17, and 6 μM, respectively; initial degradation rates were 0.015, 0.023, and 0.029 nmol/min·mg protein, respectively. This indicates that for the first time an aerobic enzyme can degrade mixtures of all chlorinated ethenes, including the once - so it was believed - completely recalcitrant PCE. © Springer-Verlag 2001.
DOI10.1007/s002530100650
URLView the original
Language英語
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Cited Times [WOS]:56   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.Jeonju University
2.Hanyang University
3.Università degli Studi dell'Insubria
4.University of Connecticut
5.Università degli Studi di Milano
Recommended Citation
GB/T 7714
Shim H.,Ryoo D.,Barbieri P.,et al. Aerobic degradation of mixtures of tetrachloroethylene, trichloroethylene, dichloroethylenes, and vinyl chloride by toluene-o-xylene monooxygenase of Pseudomonas stutzeri OX1[J]. Applied Microbiology and Biotechnology,2001,56(1-2):265-269.
APA Shim H.,Ryoo D.,Barbieri P.,&Wood T.K..(2001).Aerobic degradation of mixtures of tetrachloroethylene, trichloroethylene, dichloroethylenes, and vinyl chloride by toluene-o-xylene monooxygenase of Pseudomonas stutzeri OX1.Applied Microbiology and Biotechnology,56(1-2),265-269.
MLA Shim H.,et al."Aerobic degradation of mixtures of tetrachloroethylene, trichloroethylene, dichloroethylenes, and vinyl chloride by toluene-o-xylene monooxygenase of Pseudomonas stutzeri OX1".Applied Microbiology and Biotechnology 56.1-2(2001):265-269.
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