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Construction of a microfluidic chip, using dried-down reagents, for LATE-PCR amplification and detection of single-stranded DNA
Yanwei Jia1,2; Pui-In Mak2; Conner Massey1; Rui P. Martins2,3; Lawrence J. Wangh1
2013-12-07
Source PublicationLab on a Chip
ISSN1473-0197
Volume13Issue:23Pages:4635-4641
Abstract

LATE-PCR is an advanced form of non-symmetric PCR that efficiently generates single-stranded DNA which can readily be characterized at the end of amplification by hybridization to low-temperature fluorescent probes. We demonstrate here for the first time that monoplex and duplex LATE-PCR amplification and probe target hybridization can be carried out in double layered PDMS microfluidics chips containing dried reagents. Addition of a set of reagents during dry down overcomes the common problem of single-stranded oligonucleotide binding to PDMS. These proof-of-principle results open the way to construction of inexpensive point-of-care devices that take full advantage of the analytical power of assays built using LATE-PCR and low-temperature probes. 

DOI10.1039/c3lc51049b
URLView the original
Indexed BySCIE
Language英語English
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry ; Science & Technology - Other Topics
WOS SubjectBiochemical Research Methods ; Chemistry, Multidisciplinary ; Chemistry, Analytical ; Nanoscience & Nanotechnology
WOS IDWOS:000326983300018
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Cited Times [WOS]:9   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionDEPARTMENT OF ELECTRICAL AND COMPUTER ENGINEERING
Corresponding AuthorLawrence J. Wangh
Affiliation1.Department of Biology, Brandeis University, 415 South Street, Waltham, MA
2.State-Key Laboratory of Analog and Mixed-Signal and FST-ECE, University of Macau, Avenida Padre Tomás Pereira, Taipa, Macao, China
3.On leave from Instituto Superior Técnico, Technical University of Lisbon, Portugal.
First Author AffilicationUniversity of Macau
Recommended Citation
GB/T 7714
Yanwei Jia,Pui-In Mak,Conner Massey,et al. Construction of a microfluidic chip, using dried-down reagents, for LATE-PCR amplification and detection of single-stranded DNA[J]. Lab on a Chip,2013,13(23):4635-4641.
APA Yanwei Jia,Pui-In Mak,Conner Massey,Rui P. Martins,&Lawrence J. Wangh.(2013).Construction of a microfluidic chip, using dried-down reagents, for LATE-PCR amplification and detection of single-stranded DNA.Lab on a Chip,13(23),4635-4641.
MLA Yanwei Jia,et al."Construction of a microfluidic chip, using dried-down reagents, for LATE-PCR amplification and detection of single-stranded DNA".Lab on a Chip 13.23(2013):4635-4641.
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