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Efficient gene delivery and multimodal imaging by lanthanide-based upconversion nanoparticles
Lin Wang1,2; Jianhua Liu3; Yunlu Dai1,4; Qiang Yang1; Yuanxin Zhang1; Piaoping Yang2; Ziyong Cheng1; Hongzhou Lian1; Chunxia Li1; Zhiyao Hou1; Ping’an Ma1; Jun Lin1
2014-11-04
Source PublicationLangmuir
ISSN0743-7463
Volume30Issue:43Pages:13042−13051
Abstract

Nanoparticles have been explored as nonviral gene carriers for years because of the simplicity of surface modification and lack of immune response. Lanthanide-based upconversion nanoparticles (UCNPs) are becoming attractive candidates for biomedical applications in virtue of their unique optical properties and multimodality imaging ability. Here, we report a UCNPs-based structure with polyethylenimine coating for both efficient gene transfection and trimodality imaging. Cytotoxicity tests demonstrated that the nanoparticles exhibited significantly decreased cytotoxicity compared to polyethylenimine polymer. Further, in vitro studies revealed that the gene carriers are able to transfer the enhanced green fluorescence protein (EGFP) plasmid DNA into Hela cells in higher transfection efficiency than PEI. Gene silencing was also examined by delivering bcl-2 siRNA into Hela cells, resulting in significant downregulation of target bcl-2 mRNA. More importantly, we demonstrated the feasibility of upconversion gene carriers to serve as effective contrast agents for MRI/CT/UCL trimodality imaging both in vitro and in vivo. The facile fabrication process, great biocompatibility, enhanced gene transfection efficiency, and great bioimaging ability can make it promising for application in gene therapy

DOIhttp://doi.org/10.1021/la503444g
Indexed BySCI
Language英语
WOS Research AreaChemistry ; Materials Science
WOS SubjectChemistry, Multidisciplinary ; Chemistry, Physical ; Materials Science, Multidisciplinary
WOS IDWOS:000343951000038
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Cited Times [WOS]:40   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Corresponding AuthorPing’an Ma; Jun Lin
Affiliation1.State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, People’s Republic of China
2.Key Laboratory of Superlight Materials and Surface Technology, Ministry of Education, Harbin Engineering University, Harbin 150001, People’s Republic of China
3.Department of Radiology, The Second Hospital of Jilin University, Changchun 130022, People’s Republic of China
4.University of Chinese Academy of Sciences, Beijing 100049, People’s Republic of China
Recommended Citation
GB/T 7714
Lin Wang,Jianhua Liu,Yunlu Dai,et al. Efficient gene delivery and multimodal imaging by lanthanide-based upconversion nanoparticles[J]. Langmuir,2014,30(43):13042−13051.
APA Lin Wang.,Jianhua Liu.,Yunlu Dai.,Qiang Yang.,Yuanxin Zhang.,...&Jun Lin.(2014).Efficient gene delivery and multimodal imaging by lanthanide-based upconversion nanoparticles.Langmuir,30(43),13042−13051.
MLA Lin Wang,et al."Efficient gene delivery and multimodal imaging by lanthanide-based upconversion nanoparticles".Langmuir 30.43(2014):13042−13051.
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