UM
Mapping major replication origins on the rice plastid DNA
Wang Y.1; Tamura K.1; Saitoh Y.1; Sato T.3; Hidaka S.2; Tsutsumi K.-I.1
2002
Source PublicationPlant Biotechnology
ISSN13476114 13424580
Volume19Issue:1Pages:27-35
AbstractTo maintain and to differentiate into various plastid lineages, replication of the plastid DNA (ptDNA) and division of the plastid must take place. However, replication initiation of the ptDNA has been less understood. The present study describes identification of the initiation region (origin) of ptDNA replication in the rice cultured cells. RNA-primed newly replicated DNA strands pulse-labeled with bromodeoxyuridine were isolated and size-fractionated. Locations of these nascent strands on the ptDNA determined the two major origin regions around the 3′ region of each 23S rDNA in the inverted repeats (IRA and IRB). Two-dimensional agarose gel electrophoresis of the replication intermediates suggested that replication from each origin proceeds bidirectionally. This contrasted to replication by the double D - loop mechanism.
KeywordPlastid DNA Replication Replication origin Rice Two-dimensional agarose gel electrophoresis
DOI10.5511/plantbiotechnology.19.27
URLView the original
Language英語
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Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.Iwate University
2.Tohoku Agricultural Research Center, NARO
3.Tohoku University
Recommended Citation
GB/T 7714
Wang Y.,Tamura K.,Saitoh Y.,et al. Mapping major replication origins on the rice plastid DNA[J]. Plant Biotechnology,2002,19(1):27-35.
APA Wang Y.,Tamura K.,Saitoh Y.,Sato T.,Hidaka S.,&Tsutsumi K.-I..(2002).Mapping major replication origins on the rice plastid DNA.Plant Biotechnology,19(1),27-35.
MLA Wang Y.,et al."Mapping major replication origins on the rice plastid DNA".Plant Biotechnology 19.1(2002):27-35.
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