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Influences of PAX3-FKHR transfection on expression of MRF4 and differentiation of cultured human rhabdomyosarcoma RD cells
Li G.H.; Wang D.L.; Wang Y.; Chen Z.T.; Li D.Z.
2003
Source PublicationAi zheng = Aizheng = Chinese journal of cancer
ISSN1000467X
Volume22Issue:1Pages:30-34
AbstractBACKGROUND & OBJECTIVE: There were a few reports about the role of PAX3-FKHR fusion gene in the oncogenesis of alveolar rhabdomyosarcoma (ARMS); however, its effect on muscle-producing factor (MPF) is not clear. This study was designed to observe the effects of fusion gene PAX3-FKHR transfection on expression of MRF4 and differentiation of cultured human rhabdomyosarcoma RD cells. METHODS: To construct an eukaryotic expression vector of PAX3-FKHR gene; the plasmids of MRF4 cDNA and PAX3-FKHR cDNA were transfected into cultured RD cells with lipofectin methods. The changes of cells growth, morphologic and the expression of MHC and alpha-actin were observed. RESULTS: There was no significant changes in RD cells after transfection with PAX3-FKHR gene in growth rate, growth manner and the expression of MHC and alpha-actin. In MRF4 expressing RD cells after transfected with PAX3-FKHR, although the expression of MRF4 was not changed, morphologic appearance showed a low differentiation; expression of MHC and alpha-actin significantly decreased. CONCLUSION: There is no obvious influence on biological character and differentiation in RD cells with transfection of PAX3-FKHR gene; PAX3-FKHR does not inhibit the expression of MRF4, but can reverse the effect of MRF4 on promoting the differentiation of RD cell.
URLView the original
Language英語
Fulltext Access
Document TypeJournal article
CollectionUniversity of Macau
AffiliationThird Military Medical University
Recommended Citation
GB/T 7714
Li G.H.,Wang D.L.,Wang Y.,et al. Influences of PAX3-FKHR transfection on expression of MRF4 and differentiation of cultured human rhabdomyosarcoma RD cells[J]. Ai zheng = Aizheng = Chinese journal of cancer,2003,22(1):30-34.
APA Li G.H.,Wang D.L.,Wang Y.,Chen Z.T.,&Li D.Z..(2003).Influences of PAX3-FKHR transfection on expression of MRF4 and differentiation of cultured human rhabdomyosarcoma RD cells.Ai zheng = Aizheng = Chinese journal of cancer,22(1),30-34.
MLA Li G.H.,et al."Influences of PAX3-FKHR transfection on expression of MRF4 and differentiation of cultured human rhabdomyosarcoma RD cells".Ai zheng = Aizheng = Chinese journal of cancer 22.1(2003):30-34.
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