UM
Preparation and characterization of monoclonal antibody against HBV M protein by gene immunization
Ni Y.; Ni J.; Wang X.-H.; Zheng X.-J.; Wang Y.; Xiong S.-D.
2004-03-01
Source PublicationFudan University Journal of Medical Sciences
ISSN16728467
Volume31Issue:2Pages:202-205
AbstractPurpose: To generate McAb against HBV M protein by gene immunization. Methods: Eukaryotic expression recombinant pcDNA3-PreS2/S containing HBV M protein coding sequence was constructed. Female BALB/c mice were immunized with the plasmid for preparation of specific McAb. Results: The 875 bp PreS2/S gene was correctly inserted into pcDNA3. M protein could be detected in the supernatant of transfeced cells, local injected sites of musle and spleen. One hybridoma cell line 2D3 that could consistently produce anti-M protein monoclonal antibodies were obtained. Conclusions: Monoclonal antibodies against HBV M protein could be produced by gene immunization.
KeywordGene immunization Hepatitis B virus Intrasplenic immunization Monoclonal antibody
URLView the original
Language英語
Fulltext Access
Document TypeJournal article
CollectionUniversity of Macau
AffiliationFudan University
Recommended Citation
GB/T 7714
Ni Y.,Ni J.,Wang X.-H.,et al. Preparation and characterization of monoclonal antibody against HBV M protein by gene immunization[J]. Fudan University Journal of Medical Sciences,2004,31(2):202-205.
APA Ni Y.,Ni J.,Wang X.-H.,Zheng X.-J.,Wang Y.,&Xiong S.-D..(2004).Preparation and characterization of monoclonal antibody against HBV M protein by gene immunization.Fudan University Journal of Medical Sciences,31(2),202-205.
MLA Ni Y.,et al."Preparation and characterization of monoclonal antibody against HBV M protein by gene immunization".Fudan University Journal of Medical Sciences 31.2(2004):202-205.
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