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Synergistic effects of retinoic acid and tamoxifen on human breast cancer cells: Proteomic characterization
Wang Y.1; He Q.-Y.1; Chen H.2; Chiu J.-F.1
2007-01-15
Source PublicationExperimental Cell Research
ISSN00144827
Volume313Issue:2Pages:357-368
Abstract

The anti-estrogen tamoxifen and vitamin A-related compound, all-trans retinoic acid (RA), in combination act synergistically to inhibit the growth of MCF-7 human breast cancer cells. In the present study, we applied two-dimensional gel electrophoresis based proteomic approach to globally analyze this synergistic effect of RA and tamoxifen. Proteomic study revealed that multiple clusters of proteins were involved in RA and tamoxifen-induced apoptosis in MCF-7 breast cancer cells, including post-transcriptional and splicing factors, proteins related to cellular proliferation or differentiation, and proteins related to energy production and internal degradation systems. The negative growth factor-transforming growth factor β (TGFβ) was secreted by RA and/or tamoxifen treatment and was studies as a potential mediator of the synergistic effects of RA and tamoxifen in apoptosis. By comparing protein alterations in treatments of RA and tamoxifen alone or in combination to those of TGFβ treatment, or co-treatment with TGFβ inhibitor SB 431542, proteomic results showed that a number of proteins were involved in TGFβ signaling pathway. These results provide valuable insights into the mechanisms of RA and tamoxifen-induced TGFβ signaling pathway in breast cancer cells. © 2006 Elsevier Inc. All rights reserved.

KeywordApoptosis Breast Cancer Proteomics Retinoic Acid Tamoxifen Tgfβ
DOI10.1016/j.yexcr.2006.10.016
URLView the original
Indexed BySCIE
WOS Research AreaOncology ; Cell Biology
WOS SubjectOncology ; Cell Biology
WOS IDWOS:000243539000013
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Cited Times [WOS]:22   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorChiu J.-F.
Affiliation1.The University of Hong Kong
2.University of Vermont
Recommended Citation
GB/T 7714
Wang Y.,He Q.-Y.,Chen H.,et al. Synergistic effects of retinoic acid and tamoxifen on human breast cancer cells: Proteomic characterization[J]. Experimental Cell Research,2007,313(2):357-368.
APA Wang Y.,He Q.-Y.,Chen H.,&Chiu J.-F..(2007).Synergistic effects of retinoic acid and tamoxifen on human breast cancer cells: Proteomic characterization.Experimental Cell Research,313(2),357-368.
MLA Wang Y.,et al."Synergistic effects of retinoic acid and tamoxifen on human breast cancer cells: Proteomic characterization".Experimental Cell Research 313.2(2007):357-368.
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