UM
Suppression of pro-inflammatory adhesion molecules by PPAR-δ in human vascular endothelial cells
Fan Y.1; Wang Y.1; Tang Z.1; Zhang H.1; Qin X.1; Zhu Y.1; Guan Y.1; Wang X.1; Staels B.2; Chien S.3; Wang N.1
2008-02-01
Source PublicationArteriosclerosis, Thrombosis, and Vascular Biology
ISSN10795642
Volume28Issue:2Pages:315-321
AbstractOBJECTIVE - Endothelial activation is implicated in atherogenesis and diabetes. The role of peroxisome proliferator-activated receptor-δ (PPAR-δ) in endothelial activation remains poorly understood. In this study, we investigated the anti-inflammatory effect of PPAR-δ and the mechanism involved. METHODS AND RESULTS - In human umbilical vein endothelial cells (HUVECs), the synthetic PPAR-δ ligands GW0742 and GW501516 significantly inhibited tumor necrosis factor (TNF)-α-induced expression of vascular cell adhesion molecule-1 and E-selectin (assayed by real-time RT-PCR and Northern blotting), as well as the ensuing endothelial-leukocyte adhesion. Activation of PPAR-δ upregulated the expression of antioxidant genes superoxide dismutase 1, catalase, and thioredoxin and decreased reactive oxygen species production in ECs. Chromatin immunoprecipitation assays showed that GW0742 switched the association of BCL-6, a transcription repressor, from PPAR-δ to the vascular cell adhesion molecule (VCAM)-1 promoter. Small interfering RNA reduced endogenous PPAR-δ expression but potentiated the suppressive effect of GW0742 on EC activation, which suggests that the nonliganded PPAR-δ may have an opposite effect. CONCLUSIONS - We have demonstrated that ligand activation of PPAR-δ in ECs has a potent antiinflammatory effect, probably via a binary mechanism involving the induction of antioxidative genes and the release of nuclear corepressors. PPAR-δ agonists may have a potential for treating inflammatory diseases such as atherosclerosis and diabetes. © 2008 American Heart Association, Inc.
KeywordAdhesion molecules Endothelium Gene expression Nuclear receptor Reactive oxygen species
DOI10.1161/ATVBAHA.107.149815
URLView the original
Language英語
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Cited Times [WOS]:99   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.Peking University
2.Inserm
3.University of California, San Diego
4.Peking University Health Science Center
Recommended Citation
GB/T 7714
Fan Y.,Wang Y.,Tang Z.,et al. Suppression of pro-inflammatory adhesion molecules by PPAR-δ in human vascular endothelial cells[J]. Arteriosclerosis, Thrombosis, and Vascular Biology,2008,28(2):315-321.
APA Fan Y..,Wang Y..,Tang Z..,Zhang H..,Qin X..,...&Wang N..(2008).Suppression of pro-inflammatory adhesion molecules by PPAR-δ in human vascular endothelial cells.Arteriosclerosis, Thrombosis, and Vascular Biology,28(2),315-321.
MLA Fan Y.,et al."Suppression of pro-inflammatory adhesion molecules by PPAR-δ in human vascular endothelial cells".Arteriosclerosis, Thrombosis, and Vascular Biology 28.2(2008):315-321.
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