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Analysis of the interactions between the peptides from secreted human CKLF1 and heparin using capillary zone electrophoresis
Liu Y.1; Zhang S.1; Ling X.1; Li Y.1; Zhang Y.2; Han W.2; Wang Y.2
2008-08-01
Source PublicationJournal of Peptide Science
ISSN10752617 10991387
Volume14Issue:8Pages:984-988
AbstractThe Chemokine-like factor 1 (CKLF1) is a novel human cytokine and exhibits chemotactic activities on leukocytes. Two peptides named CKLF1-C27 and CKLF1-C19, were obtained from secreted CKLF1. In this study, a selective high-performance analytical method based on capillary zone electrophoresis (CZE) to investigate interactions between heparin and CKLF1-C27/CKLF1-C19 was developed. Samples containing CKLF1-C27/ CKLF1-C19 and heparin at various ratios were incubated at room temperature and then separated by CZE with Tris-acetate buffer at pH 7.2. Both qualitative and quantitative characterizations of the binding were determined. The binding constants of the interactions between CKLF1-C27/CKLF1-C19 and heparin were calculated as (3.38 ± 0.49) × 10 M and (1.10 ± 0.02) × 10 M by Scatchard analysis. To study structural requirements, CKLF1-C19pm and CKLF1-C19km have been synthesized, and their interactions with heparin have been studied by CZE. We found that the Pro or Lys to Ala substitution within the residues of CKLF1-C19 (CKLF1-C19pm or CKLF1-C19km) strongly decreased or abolished its interaction with heparin, suggesting that the residues of Pro affect the affinity of CKLF1-C19 for heparin, and the residues of Lys of CKLF1-C19 play the important role for the interaction of CKLF1-C19 and heparin, respectively. The methodology presented should be generally applicable to study peptides and heparin interactions quantitatively and qualitatively. Copyright © 2008 European Peptide Society and John Wiley & Sons, Ltd.
KeywordCapillary zone electrophoresis CKLF1-C19 CKLF1-C27 Heparin Interaction Peptide
DOI10.1002/psc.1028
URLView the original
Language英語
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Cited Times [WOS]:8   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.Peking University
2.Peking University Health Science Center
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GB/T 7714
Liu Y.,Zhang S.,Ling X.,et al. Analysis of the interactions between the peptides from secreted human CKLF1 and heparin using capillary zone electrophoresis[J]. Journal of Peptide Science,2008,14(8):984-988.
APA Liu Y..,Zhang S..,Ling X..,Li Y..,Zhang Y..,...&Wang Y..(2008).Analysis of the interactions between the peptides from secreted human CKLF1 and heparin using capillary zone electrophoresis.Journal of Peptide Science,14(8),984-988.
MLA Liu Y.,et al."Analysis of the interactions between the peptides from secreted human CKLF1 and heparin using capillary zone electrophoresis".Journal of Peptide Science 14.8(2008):984-988.
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