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A chimeric multi-epitope DNA vaccine elicited specific antibody response against severe acute respiratory syndrome-associated coronavirus which attenuated the virulence of SARS-CoV in vitro
Wang X.3; Xu W.3; Tong D.3; Ni J.3; Gao H.3; Wang Y.3; Chu Y.3; Li P.1; Yang X.1; Xiong S.3
2008-08-15
Source PublicationImmunology Letters
ISSN01652478
Volume119Issue:1-2Pages:71-77
AbstractEpitope-based vaccines designed to induce antibody responses specific for severe acute respiratory syndrome-associated coronavirus (SARS-CoV) are being developed as a means for increasing vaccine potency. In this study, we identified four B cell epitopes from the spike (S) and membrane (M) protein through bioinformatics analysis and constructed a multi-epitope DNA vaccine. Intramuscular immunization of mice with this vaccine was sufficient to induce specific prime as well as a long-term memory humoral immune response to at least two candidate epitopes, S and M. A DNA prime-protein boost strategy greatly enhanced the antibody generation and the immune sera not only reacted with the lysates of SARS-CoV-infected Vero cells but also neutralized the cytopathic effect of SARS by 75% at 1:160 dilution. The novel immunogenic S protein peptide revealed in this study provides new target for SARS vaccine design; and our work indicated multi-epitope DNA vaccine as an effective means for eliciting polyvalent humoral immune response against SARS-CoV. © 2008 Elsevier B.V. All rights reserved.
KeywordAntibody DNA vaccine Epitope Prime-boost SARS-CoV
DOI10.1016/j.imlet.2008.04.005
URLView the original
Language英語
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Cited Times [WOS]:8   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.Wuhan Institute of Biological Products
2.Fudan University
3.Fudan University Shanghai Medical College
Recommended Citation
GB/T 7714
Wang X.,Xu W.,Tong D.,et al. A chimeric multi-epitope DNA vaccine elicited specific antibody response against severe acute respiratory syndrome-associated coronavirus which attenuated the virulence of SARS-CoV in vitro[J]. Immunology Letters,2008,119(1-2):71-77.
APA Wang X..,Xu W..,Tong D..,Ni J..,Gao H..,...&Xiong S..(2008).A chimeric multi-epitope DNA vaccine elicited specific antibody response against severe acute respiratory syndrome-associated coronavirus which attenuated the virulence of SARS-CoV in vitro.Immunology Letters,119(1-2),71-77.
MLA Wang X.,et al."A chimeric multi-epitope DNA vaccine elicited specific antibody response against severe acute respiratory syndrome-associated coronavirus which attenuated the virulence of SARS-CoV in vitro".Immunology Letters 119.1-2(2008):71-77.
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