UM
Effect of 5-Aza-CdR on the proliferation of human colorectal carcinoma cell line and expression of tumor suppressor gene PGDH
Li M.-N.; Xie J.; Chang B.-M.; Wang Y.; Lan X.-Q.; Zhang Y.-H.; Cheng N.-L.
2008-08-25
Source PublicationTumor
ISSN10007431
Volume28Issue:8Pages:656-659
AbstractObjective: To explore the effect of 5-Aza-2′-deoxycytidine (5-Aza-CdR) on the molecular biological behaviors of SW480 cells and the expression of 15-hydroxyprostaglandin dehydrogenase (PGDH). Methods: Colorectal carcinoma SW480 cells were treated with 5-Aza-CdR. Cell proliferation was determined by MTT assay and plate colony formation test. The methylation status of the tumor suppressor gene PGDH was detected by methylation-specific PCR. The expression of PGDH protein was measured by Western blotting and the cell cycle was analyzed by flow cytometry. Results: It was shown that 5-Aza-CdR decreased the proliferation rate of SW480 cells compared with control group. The clony formation rate of SW480 cells decreased significantly after treatment with 5-Aza-CdR in 5 and 10μmol/L compared with the control group (35.4% and 26.5% vs 59.35%, P <0.01). The methylation degree in the promoter of PGDH gene was decreased and the expression of PGDH protein was detected after 5-Aza-CdR treatment. 5-Aza-CdR treatment induced G phase arrest of the SW480 cells. Conclusion: The hypermethylation of CpG islands in the promoter of PGDH gene results in the loss of PGDH protein expression in human colorectal carcinoma cell line. The demethylating agent 5-Aza-CdR could restore PGDH gene expression. These findings provide theoretic evidence for clinical treatment of human colorectal carcinoma with demethylation agent 5-Aza-CdR.
Keyword5-Aza-2'-deoxycytidine Colonic neoplasms DNA methylation Gene expression
DOI10.3781/j.issn.1000-7431.2008.08.005
URLView the original
Language英語
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Document TypeJournal article
CollectionUniversity of Macau
AffiliationShanxi Medical University
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GB/T 7714
Li M.-N.,Xie J.,Chang B.-M.,et al. Effect of 5-Aza-CdR on the proliferation of human colorectal carcinoma cell line and expression of tumor suppressor gene PGDH[J]. Tumor,2008,28(8):656-659.
APA Li M.-N..,Xie J..,Chang B.-M..,Wang Y..,Lan X.-Q..,...&Cheng N.-L..(2008).Effect of 5-Aza-CdR on the proliferation of human colorectal carcinoma cell line and expression of tumor suppressor gene PGDH.Tumor,28(8),656-659.
MLA Li M.-N.,et al."Effect of 5-Aza-CdR on the proliferation of human colorectal carcinoma cell line and expression of tumor suppressor gene PGDH".Tumor 28.8(2008):656-659.
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