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ATP-binding-cassette transporters and fluconazole-resistance of clinical Candida albicans strains
Sun W.-W.2; Gao P.-H.2; Wang Y.1; Jiang Y.-Y.2
2008-09-01
Source PublicationAcademic Journal of Second Military Medical University
ISSN0258879X
Volume29Issue:9Pages:1038-1041
AbstractObjective: To explore the relationship between CDR1 and CDR2 expression and fluconazole-resistance in clinical Candida albicans strains, so as to explore the mechanism of drug-resistance in Candida albicans. Methods: The minimal inhibitory concentrations (MICs) of clinical Candida albicans strains to fluconazole were determined by broth microdilution method. The total RNA of fluconazole-susceptible and fluconazole-resistant Candida albicans strains were extracted, and the expression of the CDR1 and CDR2 genes was examined by real-time RT-PCR. The efflux of Rhodamine 6G was determined in the strains highly expressing CDR1 and CDR2 genes. Results: The incidences of CDR1 and (or) CDR2 overexpression in fluconazole-resistant strains were significantly higher than those in the fluconazole-sensitive stains. The efflux of Rhodamine 6G was significantly increased in the fluconazole-resistant strains when glucose was added, and overexpression of CDR1 and CDR2 were observed in theses strains. Some resistant strains showed no overexpression of CDR1 and CDR2. Conclusion: Fluconazole-resistance in clinical Candida albicans strains is related to the overexpression of ABC transporter proteins. Overexpression of ABC transporters can increase the efflux of drugs and therefore lead to drug-resistance. Other mechanisms may also participate in the development of drug resistance in the clinical Candida albicans strains.
KeywordABC transporter proteins Candida albicans Fluconazole Fungal drug resistance Rohdamine 6G
DOI10.3724/SP.J.1008.2008.01038
URLView the original
Language英語
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Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.Changhai Hospital
2.Second Military Medical University
Recommended Citation
GB/T 7714
Sun W.-W.,Gao P.-H.,Wang Y.,et al. ATP-binding-cassette transporters and fluconazole-resistance of clinical Candida albicans strains[J]. Academic Journal of Second Military Medical University,2008,29(9):1038-1041.
APA Sun W.-W.,Gao P.-H.,Wang Y.,&Jiang Y.-Y..(2008).ATP-binding-cassette transporters and fluconazole-resistance of clinical Candida albicans strains.Academic Journal of Second Military Medical University,29(9),1038-1041.
MLA Sun W.-W.,et al."ATP-binding-cassette transporters and fluconazole-resistance of clinical Candida albicans strains".Academic Journal of Second Military Medical University 29.9(2008):1038-1041.
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