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The Suppression of Medium Acidosis Improves the Maintenance and Differentiation of Human Pluripotent Stem Cells at High Density in Defined Cell Culture Medium
Liu, Weiwei1; Ren, Zhili1; Lu, Kai1; Song, Chengcheng1; Cheung, Edwin Chong Wing1; Zhou, Zhou2; Chen, Guokai1
2018
Source PublicationINTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
ISSN1449-2288
Volume14Issue:5Pages:485-496
Abstract

Cell density has profound impacts on the cell culture practices of human pluripotent stem cells. The regulation of cell growth, cell death, pluripotency and differentiation converge at high density, but it is largely unknown how different regulatory mechanisms act at this stage. We use a chemically defined medium to systemically examine cellular activities and the impact of medium components in high-density culture. We show that medium acidosis is the main factor that alters cell cycle, gene expression and cellular metabolism at high cell density. The low medium pH leads to inhibition of glucose consumption, cell cycle arrest, and subsequent cell death. At high cell density, the suppression of medium acidosis with sodium bicarbonate (NaHCO3) significantly increases culture capacity for stem cell survival, derivation, maintenance and differentiation. Our study provides a simple and effective tool to improve stem cell maintenance and applications.

KeywordHuman Pluripotent Stem Cells Acidosis Glycolysis Cell Death Differentiation
DOI10.7150/ijbs.24681
URLView the original
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics
WOS SubjectBiochemistry & Molecular Biology ; Biology
WOS IDWOS:000430149900001
PublisherIVYSPRING INT PUBL
The Source to ArticleWOS
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Cited Times [WOS]:4   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Affiliation1.University of Macau, Faculty of Health Sciences, Taipa, Macau
2.State Key Laboratory of Cardiovascular Disease, Beijing Key Laboratory for Molecular Diagnostics of Cardiovascular Diseases, Diagnostic Laboratory Service, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100037, China
First Author AffilicationFaculty of Health Sciences
Recommended Citation
GB/T 7714
Liu, Weiwei,Ren, Zhili,Lu, Kai,et al. The Suppression of Medium Acidosis Improves the Maintenance and Differentiation of Human Pluripotent Stem Cells at High Density in Defined Cell Culture Medium[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES,2018,14(5):485-496.
APA Liu, Weiwei.,Ren, Zhili.,Lu, Kai.,Song, Chengcheng.,Cheung, Edwin Chong Wing.,...&Chen, Guokai.(2018).The Suppression of Medium Acidosis Improves the Maintenance and Differentiation of Human Pluripotent Stem Cells at High Density in Defined Cell Culture Medium.INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES,14(5),485-496.
MLA Liu, Weiwei,et al."The Suppression of Medium Acidosis Improves the Maintenance and Differentiation of Human Pluripotent Stem Cells at High Density in Defined Cell Culture Medium".INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES 14.5(2018):485-496.
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