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MicroRNA-19a mediates the suppressive effect of laminar flow on cyclin D1 expression in human umbilical vein endothelial cells
Qin X.2; Wang X.2; Wang Y.2; Tang Z.2; Cui Q.2; Xi J.3; Li Y.-S.J.1; Chien S.1; Wang N.2
2010-02-16
Source PublicationProceedings of the National Academy of Sciences of the United States of America
ISSN00278424 10916490
Volume107Issue:7Pages:3240-3244
AbstractEndothelial cells (ECs) respond to changes in mechanical forces, leading to the modulation of signaling networks and cell function; an example is the inhibition of EC proliferation by steady laminar flow. MicroRNAs (miRs) are short noncoding 20-22 nucleotide RNAs that negatively regulate the expression of target genes at the posttranscriptional level. This study demonstrates that miRs are involved in the flow regulation of gene expression in ECs. With the use of microRNA chip array, we found that laminar shear stress (12 dyn/cm , 12 h) regulated the EC expression of manymiRs, including miR-19a.Wefurther showed that stable transfection of miR-19a significantly decreased the expression of a reporter gene controlled by a conserved 3′-untranslated region of the cyclinD1 gene and also the protein level of cyclin D1, leading to an arrest of cell cycle at G1/S transition. Laminar flow suppressed cyclin D1 protein level, and this suppressive effect was diminished when the endogenous miR-19a was inhibited. In conclusion, we demonstrated that miR-19a plays an important role in the flow regulation of cyclin D1 expression. These results revealed a mechanism by which mechanical forces modulate endothelial gene expression.
KeywordCell cycle Endothelium Flow Gene expression Noncoding RNA
DOI10.1073/pnas.0914882107
URLView the original
Language英語
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被引频次[WOS]:156   [WOS记录]     [WOS相关记录]
Document TypeJournal article
专题University of Macau
Affiliation1.University of California, San Diego
2.Peking University Health Science Center
3.Peking University
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Qin X.,Wang X.,Wang Y.,et al. MicroRNA-19a mediates the suppressive effect of laminar flow on cyclin D1 expression in human umbilical vein endothelial cells[J]. Proceedings of the National Academy of Sciences of the United States of America,2010,107(7):3240-3244.
APA Qin X..,Wang X..,Wang Y..,Tang Z..,Cui Q..,...&Wang N..(2010).MicroRNA-19a mediates the suppressive effect of laminar flow on cyclin D1 expression in human umbilical vein endothelial cells.Proceedings of the National Academy of Sciences of the United States of America,107(7),3240-3244.
MLA Qin X.,et al."MicroRNA-19a mediates the suppressive effect of laminar flow on cyclin D1 expression in human umbilical vein endothelial cells".Proceedings of the National Academy of Sciences of the United States of America 107.7(2010):3240-3244.
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