UM
Triptolide inhibits cytokine-induced activation of retro-ocular fibroblasts from patients with graves' ophthalmopathy
Yan S.-X.1; Wang Y.1; Bin Z.2; Zhi-Hai F.1; Jiu-Sheng L.1; Ya-Ru S.1; Yan-Jie L.1
2011-10-30
Source PublicationJournal of Medicinal Plant Research
ISSN19960875
Volume5Issue:24Pages:5789-5795
AbstractTo explore the effects of Triptolide which has immunosuppressive and anti-inflammatory properties on the expression of human leucocyte antigen-DR (HLA-DR), intercellular adhesion molecule-1 (ICAM-1), CD40 and Fas enduced by cytokines and the synthesis of hyaluronic acid (HA) on cultured retro-ocular fibroblasts (RFs) from patients with Graves' ophthalmopathy. Cultured RFs were incubated with IL-1 (100 U/mL) or TNF-α (100 U/mL) in the presence of Triptolide. Flow cytometry was used to investigate the amount of HLA-DR, ICAM-1, CD40 and Fas. HA synthesis was measured by radioimmunoassay. At base conditions, the percentage of positive cells of HLA-DR, ICAM-1 and CD40 on RFs were 6.70 ± 3.06%, 14.89 ± 11.67% and 5.29 ± 3.02%, and the synthesis of HA was 337.8 ± 42.7 ng/ml. IL-1 or TNF-α significantly enhanced the amount of HLA-DR, ICAM-1 and CD40 and HA synthesis. Triptolide 0.01 μg/L had little effect on cytokine- induced HLA-DR, ICAM-1 and CD40 amounts, as well as HA synthesis. When the concentration ranged from 0.1 to 10 μg/L, Triptolide inhibited cytokine- induced RFs activation in a dose-dependent manner. Triptolide itself also induced Fas expression on RFs in a dose-dependent manner. Triptolide could inhibit cytokines-induced activation of RFs derived from patients with Graves' ophthalmopathy. © 2011 Academic Journals.
KeywordCytokine Fibroblasts Graves'ophthalmopathy Retro-ocular Triptolide
URLView the original
Language英語
Fulltext Access
Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.First Affiliated Hospital of Zhengzhou University
2.Henan College of Traditional Chinese Medicine
Recommended Citation
GB/T 7714
Yan S.-X.,Wang Y.,Bin Z.,et al. Triptolide inhibits cytokine-induced activation of retro-ocular fibroblasts from patients with graves' ophthalmopathy[J]. Journal of Medicinal Plant Research,2011,5(24):5789-5795.
APA Yan S.-X..,Wang Y..,Bin Z..,Zhi-Hai F..,Jiu-Sheng L..,...&Yan-Jie L..(2011).Triptolide inhibits cytokine-induced activation of retro-ocular fibroblasts from patients with graves' ophthalmopathy.Journal of Medicinal Plant Research,5(24),5789-5795.
MLA Yan S.-X.,et al."Triptolide inhibits cytokine-induced activation of retro-ocular fibroblasts from patients with graves' ophthalmopathy".Journal of Medicinal Plant Research 5.24(2011):5789-5795.
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