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Erythropoietin and hypoxia increase erythropoietin receptor and nitric oxide levels in lung microvascular endothelial cells
Beleslin-Cokic B.B.1; Cokic V.P.3; Wang L.2; Piknova B.2; Teng R.2; Schechter A.N.2; Noguchi C.T.2
2011-05-01
Source PublicationCytokine
ISSN10434666
Volume54Issue:2Pages:129-135
Abstract

Acute lung exposure to low oxygen results in pulmonary vasoconstriction and redistribution of blood flow. We used human microvascular endothelial cells from lung (HMVEC-L) to study the acute response to oxygen stress. We observed that hypoxia and erythropoietin (EPO) increased erythropoietin receptor (EPOR) gene expression and protein level in HMVEC-L. In addition, EPO dose- and time-dependently stimulated nitric oxide (NO) production. This NO stimulation was evident despite hypoxia induced reduction of endothelial NO synthase (eNOS) gene expression. Western blot of phospho-eNOS (serine1177) and eNOS and was significantly induced by hypoxia but not after EPO treatment. However, iNOS increased at hypoxia and with EPO stimulation compared to normal oxygen tension. In accordance with our previous results of NO induction by EPO at low oxygen tension in human umbilical vein endothelial cells and bone marrow endothelial cells, these results provide further evidence in HMVEC-L for EPO regulation of NO production to modify the effects of hypoxia and cause compensatory vasoconstriction. © 2011 Elsevier Ltd.

KeywordErythropoietin Erythropoietin Receptor Hmvec-l Hypoxia No
DOI10.1016/j.cyto.2011.01.015
URLView the original
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Cell Biology ; Immunology
WOS SubjectBiochemistry & Molecular Biology ; Cell Biology ; Immunology
WOS IDWOS:000290063200005
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Cited Times [WOS]:25   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Affiliation1.Clinical Center of Serbia
2.National Institute of Diabetes and Digestive and Kidney Diseases
3.Institute of Medical Research Yugoslavia Serbia
Recommended Citation
GB/T 7714
Beleslin-Cokic B.B.,Cokic V.P.,Wang L.,et al. Erythropoietin and hypoxia increase erythropoietin receptor and nitric oxide levels in lung microvascular endothelial cells[J]. Cytokine,2011,54(2):129-135.
APA Beleslin-Cokic B.B..,Cokic V.P..,Wang L..,Piknova B..,Teng R..,...&Noguchi C.T..(2011).Erythropoietin and hypoxia increase erythropoietin receptor and nitric oxide levels in lung microvascular endothelial cells.Cytokine,54(2),129-135.
MLA Beleslin-Cokic B.B.,et al."Erythropoietin and hypoxia increase erythropoietin receptor and nitric oxide levels in lung microvascular endothelial cells".Cytokine 54.2(2011):129-135.
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