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Aspergillus myosin-V supports polarized growth in the absence of microtubule-based transport
Zhang J.2; Tan K.4; Wu X.5; Chen G.2; Sun J.5; Reck-Peterson S.L.4; Hammer J.A.5; Xiang X.2
2011-12-14
Source PublicationPLoS ONE
ISSN19326203
Volume6Issue:12
Abstract

In the filamentous fungus Aspergillus nidulans, both microtubules and actin filaments are important for polarized growth at the hyphal tip. Less clear is how different microtubule-based and actin-based motors work together to support this growth. Here we examined the role of myosin-V (MYOV) in hyphal growth. MYOV-depleted cells form elongated hyphae, but the rate of hyphal elongation is significantly reduced. In addition, although wild type cells without microtubules still undergo polarized growth, microtubule disassembly abolishes polarized growth in MYOV-depleted cells. Thus, MYOV is essential for polarized growth in the absence of microtubules. Moreover, while a triple kinesin null mutant lacking kinesin-1 (KINA) and two kinesin-3s (UNCA and UNCB) undergoes hyphal elongation and forms a colony, depleting MYOV in this triple mutant results in lethality due to a severe defect in polarized growth. These results argue that MYOV, through its ability to transport secretory cargo, can support a significant amount of polarized hyphal tip growth in the absence of any microtubule-based transport. Finally, our genetic analyses also indicate that KINA (kinesin-1) rather than UNCA (kinesin-3) is the major kinesin motor that supports polarized growth in the absence of MYOV.

DOI10.1371/journal.pone.0028575
URLView the original
Indexed BySCI
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000298369100054
Fulltext Access
Citation statistics
Cited Times [WOS]:23   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Affiliation1.River Hill High School
2.Uniformed Services University of the Health Sciences
3.Yale University
4.Harvard Medical School
5.National Heart, Lung, and Blood Institute
Recommended Citation
GB/T 7714
Zhang J.,Tan K.,Wu X.,et al. Aspergillus myosin-V supports polarized growth in the absence of microtubule-based transport[J]. PLoS ONE,2011,6(12).
APA Zhang J..,Tan K..,Wu X..,Chen G..,Sun J..,...&Xiang X..(2011).Aspergillus myosin-V supports polarized growth in the absence of microtubule-based transport.PLoS ONE,6(12).
MLA Zhang J.,et al."Aspergillus myosin-V supports polarized growth in the absence of microtubule-based transport".PLoS ONE 6.12(2011).
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