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Lis1 is an initiation factor for dynein-driven organelle transport
Egan M.J.; Tan K.; Reck-Peterson S.L.
2012-06-25
Source PublicationJournal of Cell Biology
ISSN00219525 15408140
Volume197Issue:7Pages:971-982
Abstract

The molecular motor cytoplasmic dynein is responsible for most minus-end-directed, microtubule-based transport in eukaryotic cells. It is especially important in neurons, where defects in microtubule-based motility have been linked to neurological diseases. For example, lissencephaly is caused by mutations in the dynein-associated protein Lis1. In this paper, using the long, highly polarized hyphae of the filamentous fungus Aspergillus nidulans, we show that three morphologically and functionally distinct dynein cargos showed transport defects in the genetic absence of Lis1/nudF, raising the possibility that Lis1 is ubiquitously used for dynein-based transport. Surprisingly, both dynein and its cargo moved at normal speeds in the absence of Lis1 but with reduced frequency. Moreover, Lis1, unlike dynein and dynactin, was absent from moving dynein cargos, further suggesting that Lis1 is not required for dynein-based cargo motility once it has commenced. Based on these observations, we propose that Lis1 has a general role in initiating dynein-driven motility. © 2012 Egan et al.

DOI10.1083/jcb.201112101
URLView the original
Indexed BySCI
WOS Research AreaCell Biology
WOS SubjectCell Biology
WOS IDWOS:000305807000012
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Citation statistics
Cited Times [WOS]:107   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
AffiliationHarvard Medical School
Recommended Citation
GB/T 7714
Egan M.J.,Tan K.,Reck-Peterson S.L.. Lis1 is an initiation factor for dynein-driven organelle transport[J]. Journal of Cell Biology,2012,197(7):971-982.
APA Egan M.J.,Tan K.,&Reck-Peterson S.L..(2012).Lis1 is an initiation factor for dynein-driven organelle transport.Journal of Cell Biology,197(7),971-982.
MLA Egan M.J.,et al."Lis1 is an initiation factor for dynein-driven organelle transport".Journal of Cell Biology 197.7(2012):971-982.
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