UM  > 健康科學學院
Conservation of an RNA regulatory map between Drosophila and mammals
Brooks A.N.1; Yang L.2; Duff M.O.2; Hansen K.D.1; Park J.W.2; Dudoit S.1; Brenner S.E.1; Graveley B.R.2
2011-02-01
Source PublicationGenome Research
ISSN10889051 15495469
Volume21Issue:2Pages:193-202
Abstract

Alternative splicing is generally controlled by proteins that bind directly to regulatory sequence elements and either activate or repress splicing of adjacent splice sites in a target pre-mRNA. Here, we have combined RNAi and mRNA-seq to identify exons that are regulated by Pasilla (PS), the Drosophila melanogaster ortholog of mammalian NOVA1 and NOVA2. We identified 405 splicing events in 323 genes that are significantly affected upon depletion of ps, many of which were annotated as being constitutively spliced. The sequence regions upstream and within PS-repressed exons and downstream from PS-activated exons are enriched for YCAY repeats, and these are consistent with the location of these motifs near NOVA-regulated exons in mammals. Thus, the RNA regulatory map of PS and NOVA1/2 is highly conserved between insects and mammals despite the fact that the target gene orthologs regulated by PS and NOVA1/2 are almost entirely nonoverlapping. This observation suggests that the regulatory codes of individual RNA binding proteins may be nearly immutable, yet the regulatory modules controlled by these proteins are highly evolvable. © 2011 by Cold Spring Harbor Laboratory Press.

DOI10.1101/gr.108662.110
URLView the original
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Genetics & Heredity
WOS SubjectBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Genetics & Heredity
WOS IDWOS:000286804100005
Fulltext Access
Citation statistics
Cited Times [WOS]:118   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Affiliation1.University of California, Berkeley
2.University of Connecticut Health Center
Recommended Citation
GB/T 7714
Brooks A.N.,Yang L.,Duff M.O.,et al. Conservation of an RNA regulatory map between Drosophila and mammals[J]. Genome Research,2011,21(2):193-202.
APA Brooks A.N..,Yang L..,Duff M.O..,Hansen K.D..,Park J.W..,...&Graveley B.R..(2011).Conservation of an RNA regulatory map between Drosophila and mammals.Genome Research,21(2),193-202.
MLA Brooks A.N.,et al."Conservation of an RNA regulatory map between Drosophila and mammals".Genome Research 21.2(2011):193-202.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Brooks A.N.]'s Articles
[Yang L.]'s Articles
[Duff M.O.]'s Articles
Baidu academic
Similar articles in Baidu academic
[Brooks A.N.]'s Articles
[Yang L.]'s Articles
[Duff M.O.]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Brooks A.N.]'s Articles
[Yang L.]'s Articles
[Duff M.O.]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.