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CDK phosphorylates the polarisome scaffold Spa2 to maintain its localization at the site of cell growth
Wang H.2; Huang Z.-X.2; Au Yong J.Y.2; Zou H.2; Zeng G.2; Gao J.2; Wang Y.2; Wong A.H.-H.1; Wang Y.2
2016-07-01
Source PublicationMolecular Microbiology
ISSN13652958 0950382X
Volume101Issue:2Pages:250-264
AbstractPolarisome is a protein complex that plays an important role in polarized growth in fungi by assembling actin cables towards the site of cell growth. For proper morphogenesis, the polarisome must localize to the right place at the right time. However, the mechanisms that control polarisome localization remain poorly understood. In this study, using the polymorphic fungus Candida albicans as a model, we have discovered that the cyclin-dependent kinase (CDK) Cdc28 phosphorylates the polarisome scaffold protein Spa2 to govern polarisome localization during both yeast and hyphal growth. In a yeast cell cycle, Cdc28-Clb2 phosphorylates Spa2 and controls the timing of polarisome translocation from the bud tip to the bud neck. And during hyphal development, Cdc28-Clb2 and the hyphal-specific Cdc28-Hgc1 cooperate to enhance Spa2 phosphorylation to maintain the polarisome at the hyphal tip. Blocking the CDK phosphorylation causes premature tip-to-neck translocation of Spa2 during yeast growth and inappropriate septal localization of Spa2 in hyphae and abnormal hyphal morphology under certain inducing conditions. Together, our results generate new insights into the mechanisms by which fungi regulate polarisome localization in the control of polarized growth.
DOI10.1111/mmi.13386
URLView the original
Language英語
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Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.Universidade de Macau
2.A-Star, Institute of Molecular and Cell Biology
3.Yong Loo Lin School of Medicine
Recommended Citation
GB/T 7714
Wang H.,Huang Z.-X.,Au Yong J.Y.,et al. CDK phosphorylates the polarisome scaffold Spa2 to maintain its localization at the site of cell growth[J]. Molecular Microbiology,2016,101(2):250-264.
APA Wang H..,Huang Z.-X..,Au Yong J.Y..,Zou H..,Zeng G..,...&Wang Y..(2016).CDK phosphorylates the polarisome scaffold Spa2 to maintain its localization at the site of cell growth.Molecular Microbiology,101(2),250-264.
MLA Wang H.,et al."CDK phosphorylates the polarisome scaffold Spa2 to maintain its localization at the site of cell growth".Molecular Microbiology 101.2(2016):250-264.
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