UM  > 健康科學學院
Epigenetic priming of enhancers predicts developmental competence of hESC-derived endodermal lineage intermediates
Wang A.1; Yue F.4; Li Y.3; Xie R.1; Harper T.1; Patel N.A.1; Muth K.1; Palmer J.1; Qiu Y.3; Wang J.1; Lam D.K.1; Raum J.C.5; Stoffers D.A.5; Ren B.3; Sander M.1
2015
Source PublicationCell Stem Cell
ISSN18759777 19345909
Volume16Issue:4Pages:386-399
Abstract

Embryonic development relies on the capacity of progenitor cells to appropriately respond to inductive cues, a cellular property known as developmental competence. Here, we report that epigenetic priming of enhancers signifies developmental competence during endodermal lineage diversification. Chromatin mapping during pancreatic and hepatic differentiation of human embryonic stem cells revealed the en masse acquisition of a poised chromatin state at enhancers specific to endoderm-derived cell lineages in gut tube intermediates. Experimentally, the acquisition of this poised enhancer state predicts the ability of endodermal intermediates to respond to inductive signals. Furthermore, these enhancers are first recognized by the pioneer transcription factors FOXA1 and FOXA2 when competence is acquired, while subsequent recruitment of lineage-inductive transcription factors, such as PDX1, leads to enhancer and target gene activation. Together, our results identify the acquisition of a poised chromatin state at enhancers as a mechanism by which progenitor cells acquire developmental competence.

DOI10.1016/j.stem.2015.02.013
URLView the original
Indexed BySCI
WOS Research AreaCell Biology
WOS SubjectCell & Tissue Engineering ; Cell Biology
WOS IDWOS:000352228600011
Fulltext Access
Citation statistics
Cited Times [WOS]:82   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Affiliation1.University of California, San Diego
2.Sanford Consortium for Regenerative Medicine
3.Ludwig Institute for Cancer Research San Diego
4.Pennsylvania State University
5.University of Pennsylvania
Recommended Citation
GB/T 7714
Wang A.,Yue F.,Li Y.,et al. Epigenetic priming of enhancers predicts developmental competence of hESC-derived endodermal lineage intermediates[J]. Cell Stem Cell,2015,16(4):386-399.
APA Wang A..,Yue F..,Li Y..,Xie R..,Harper T..,...&Sander M..(2015).Epigenetic priming of enhancers predicts developmental competence of hESC-derived endodermal lineage intermediates.Cell Stem Cell,16(4),386-399.
MLA Wang A.,et al."Epigenetic priming of enhancers predicts developmental competence of hESC-derived endodermal lineage intermediates".Cell Stem Cell 16.4(2015):386-399.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wang A.]'s Articles
[Yue F.]'s Articles
[Li Y.]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wang A.]'s Articles
[Yue F.]'s Articles
[Li Y.]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wang A.]'s Articles
[Yue F.]'s Articles
[Li Y.]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.