UM
Identification of a Critical and Conformational Neutralizing Epitope in Human Adenovirus Type 4 Hexon
Tian, Xingui; Qiu, Hongling; Zhou, Zhichao; Wang, Shouli; Fan, Ye; Li, Xiao; Chu, Ruiai; Li, Haitao; Zhou, Rong; Wang, Hui
2018-01
Source PublicationJOURNAL OF VIROLOGY
ISSN0022-538X
Volume92Issue:2
AbstractHuman adenovirus type 4 (HAdV-4) is an epidemic virus that contributes to serious acute respiratory disease (ARD) in both pediatric and adult patients. However, no licensed drug or vaccine is currently available to the civilian population. The identification of neutralizing epitopes of HAdV-4 should allow the development of a novel antiviral vaccine and a novel gene transfer vector, and an effective neutralizing monoclonal antibody (MAb) will be useful in developing appropriate antiviral drugs. In this study, we report that MAb MN4b shows strong neutralizing activity against HAdV-4. MN4b recognizes a conformational epitope (418AGSEK422) within hypervariable region 7 (HVR7). Mutations within this site permitted HAdV-4 mutants to escape neutralization by MN4b and to resist neutralization by animal and human anti-HAdV-4 sera. A recombinant virus, rAd3-A4R7-1, containing the identified neutralizing epitope in the HVR7 region of HAdV-3 hexon, successfully induced antiserum that inhibited HAdV-4 infection. These results indicate that a small surface loop of HAdV-4 hexon is a critical neutralization epitope for this virus. The generation of MN4b and the identification of this neutralizing epitope may be useful in developing therapeutic treatment, a subunit vaccine, and a novel vector that can escape preexisting neutralization for HAdV-4.
Keywordadenovirus type 4 conformational epitope neutralizing monoclonal antibody acute respiratory disease adenoviral vector
DOI10.1128/JVI.01643-17
URLView the original
Indexed BySCI
Language英语
WOS Research AreaVirology
WOS SubjectVirology
WOS IDWOS:000419042000021
PublisherAMER SOC MICROBIOLOGY
The Source to ArticleWOS
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Cited Times [WOS]:4   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Recommended Citation
GB/T 7714
Tian, Xingui,Qiu, Hongling,Zhou, Zhichao,et al. Identification of a Critical and Conformational Neutralizing Epitope in Human Adenovirus Type 4 Hexon[J]. JOURNAL OF VIROLOGY,2018,92(2).
APA Tian, Xingui.,Qiu, Hongling.,Zhou, Zhichao.,Wang, Shouli.,Fan, Ye.,...&Wang, Hui.(2018).Identification of a Critical and Conformational Neutralizing Epitope in Human Adenovirus Type 4 Hexon.JOURNAL OF VIROLOGY,92(2).
MLA Tian, Xingui,et al."Identification of a Critical and Conformational Neutralizing Epitope in Human Adenovirus Type 4 Hexon".JOURNAL OF VIROLOGY 92.2(2018).
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