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Clathrin and AP-1 regulate apical polarity and lumen formation during C. elegans tubulogenesis
Gobel V.1; Zhang H.1; Kim A.1; Abraham N.1; Khan L.A.1; Hall D.H.2; Fleming J.T.1
2012-06-01
Source PublicationDevelopment
ISSN09501991 14779129
Volume139Issue:11Pages:2071-2083
Abstract

Clathrin coats vesicles in all eukaryotic cells and has a well-defined role in endocytosis, moving molecules away from the plasma membrane. Its function on routes towards the plasma membrane was only recently appreciated and is thought to be limited to basolateral transport. Here, an unbiased RNAi-based tubulogenesis screen identifies a role of clathrin (CHC-1) and its AP-1 adaptor in apical polarity during de novo lumenal membrane biogenesis in the C. elegans intestine. We show that CHC-1/AP-1-mediated polarized transport intersects with a sphingolipid-dependent apical sorting process. Depleting each presumed trafficking component mislocalizes the same set of apical membrane molecules basolaterally, including the polarity regulator PAR-6, and generates ectopic lateral lumens. GFP::CHC-1 and BODIPY-ceramide vesicles associate perinuclearly and assemble asymmetrically at polarized plasma membrane domains in a co-dependent and AP-1-dependent manner. Based on these findings, we propose a trafficking pathway for apical membrane polarity and lumen morphogenesis that implies: (1) a clathrin/AP-1 function on an apically directed transport route; and (2) the convergence of this route with a sphingolipid-dependent apical trafficking path. © 2012. Published by The Company of Biologists Ltd.

KeywordAp-1 Caenorhabditis Elegans Clathrin Polarity Sphingolipids Tubulogenesis
DOI10.1242/dev.077347
URLView the original
Indexed BySCI
WOS Research AreaDevelopmental Biology
WOS SubjectDevelopmental Biology
WOS IDWOS:000303914300023
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Cited Times [WOS]:33   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Affiliation1.Massachusetts General Hospital
2.Albert Einstein College of Medicine of Yeshiva University
Recommended Citation
GB/T 7714
Gobel V.,Zhang H.,Kim A.,et al. Clathrin and AP-1 regulate apical polarity and lumen formation during C. elegans tubulogenesis[J]. Development,2012,139(11):2071-2083.
APA Gobel V..,Zhang H..,Kim A..,Abraham N..,Khan L.A..,...&Fleming J.T..(2012).Clathrin and AP-1 regulate apical polarity and lumen formation during C. elegans tubulogenesis.Development,139(11),2071-2083.
MLA Gobel V.,et al."Clathrin and AP-1 regulate apical polarity and lumen formation during C. elegans tubulogenesis".Development 139.11(2012):2071-2083.
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