UM  > 健康科學學院
Intracellular lumen extension requires ERM-1-dependent apical membrane expansion and AQP-8-mediated flux
Khan L.A.2; Zhang H.2; Abraham N.2; Sun L.4; Fleming J.T.2; Buechner M.3; Hall D.H.4; Gobel V.2
2013-02-01
Source PublicationNature Cell Biology
ISSN14657392 14764679
Volume15Issue:2Pages:143-156
Abstract

Many unicellular tubes such as capillaries form lumens intracellularly, a process that is not well understood. Here we show that the cortical membrane organizer ERM-1 is required to expand the intracellular apical/lumenal membrane and its actin undercoat during single-cell Caenorhabditis elegans excretory canal morphogenesis. We characterize AQP-8, identified in an ERM-1-overexpression (ERM-1[++]) suppressor screen, as a canalicular aquaporin that interacts with ERM-1 in lumen extension in a mercury-sensitive manner, implicating water-channel activity. AQP-8 is transiently recruited to the lumen by ERM-1, co-localizing in peri-lumenal cuffs interspaced along expanding canals. An ERM-1[++]-mediated increase in the number of lumen-associated canaliculi is reversed by AQP-8 depletion. We propose that the ERM-1/AQP-8 interaction propels lumen extension by translumenal flux, suggesting a direct morphogenetic effect of water-channel-regulated fluid pressure. © 2013 Macmillan Publishers Limited. All rights reserved.

DOI10.1038/ncb2656
URLView the original
Indexed BySCI
WOS Research AreaCell Biology
WOS SubjectCell Biology
WOS IDWOS:000315844900016
Fulltext Access
Citation statistics
Document TypeJournal article
CollectionFaculty of Health Sciences
Affiliation1.Institute of Biophysics Chinese Academy of Sciences
2.Massachusetts General Hospital
3.University of Kansas Lawrence
4.Albert Einstein College of Medicine of Yeshiva University
Recommended Citation
GB/T 7714
Khan L.A.,Zhang H.,Abraham N.,et al. Intracellular lumen extension requires ERM-1-dependent apical membrane expansion and AQP-8-mediated flux[J]. Nature Cell Biology,2013,15(2):143-156.
APA Khan L.A..,Zhang H..,Abraham N..,Sun L..,Fleming J.T..,...&Gobel V..(2013).Intracellular lumen extension requires ERM-1-dependent apical membrane expansion and AQP-8-mediated flux.Nature Cell Biology,15(2),143-156.
MLA Khan L.A.,et al."Intracellular lumen extension requires ERM-1-dependent apical membrane expansion and AQP-8-mediated flux".Nature Cell Biology 15.2(2013):143-156.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Khan L.A.]'s Articles
[Zhang H.]'s Articles
[Abraham N.]'s Articles
Baidu academic
Similar articles in Baidu academic
[Khan L.A.]'s Articles
[Zhang H.]'s Articles
[Abraham N.]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Khan L.A.]'s Articles
[Zhang H.]'s Articles
[Abraham N.]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.