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Sumoylation in Aspergillus nidulans: sumO inactivation, overexpression and live-cell imaging
Wong K.H.1; Todd R.B.1; Oakley B.R.2; Oakley C.E.2; Hynes M.J.1; Davis M.A.1
2008-05-01
Source PublicationFungal Genetics and Biology
ISSN10871845 10960937
Volume45Issue:5Pages:728-737
Abstract

Sumoylation, the reversible covalent attachment of small ubiquitin-like modifier (SUMO) peptides has emerged as an important regulator of target protein function. In Saccharomyces cerevisiae, but not in Schizosaccharyomes pombe, deletion of the gene encoding SUMO peptides is lethal. We have characterized the SUMO-encoding gene, sumO, in the filamentous fungus Aspergillus nidulans. The sumO gene was deleted in a diploid and sumOΔ haploids were recovered. The mutant was viable but exhibited impaired growth, reduced conidiation and self-sterility. Overexpression of epitope-tagged SumO peptides revealed multiple sumoylation targets in A. nidulans and SumO overexpression resulted in greatly increased levels of protein sumoylation without obvious phenotypic consequences. Using five-piece fusion PCR, we generated a gfp-sumO fusion gene expressed from the sumO promoter for live-cell imaging of GFP-SumO and GFP-SumO-conjugated proteins. Localization of GFP-SumO is dynamic, accumulating in punctate spots within the nucleus during interphase, lost at the onset of mitosis and re-accumulating during telophase. © 2007 Elsevier Inc. All rights reserved.

KeywordCell Cycle Conidiation Fusion Pcr Sexual Development Sumo
DOI10.1016/j.fgb.2007.12.009
URLView the original
Indexed BySCI
WOS Research AreaGenetics & Heredity ; Mycology
WOS SubjectGenetics & Heredity ; Mycology
WOS IDWOS:000255153600015
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Cited Times [WOS]:24   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Affiliation1.University of Melbourne
2.Ohio State University
Recommended Citation
GB/T 7714
Wong K.H.,Todd R.B.,Oakley B.R.,et al. Sumoylation in Aspergillus nidulans: sumO inactivation, overexpression and live-cell imaging[J]. Fungal Genetics and Biology,2008,45(5):728-737.
APA Wong K.H.,Todd R.B.,Oakley B.R.,Oakley C.E.,Hynes M.J.,&Davis M.A..(2008).Sumoylation in Aspergillus nidulans: sumO inactivation, overexpression and live-cell imaging.Fungal Genetics and Biology,45(5),728-737.
MLA Wong K.H.,et al."Sumoylation in Aspergillus nidulans: sumO inactivation, overexpression and live-cell imaging".Fungal Genetics and Biology 45.5(2008):728-737.
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