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Multiple nuclear localization signals mediate nuclear localization of the GATA transcription factor AreA
Hunter C.C.4; Siebert K.S.4; Downes D.J.4; Wong K.H.5; Kreutzberger S.D.5; Fraser J.A.5; Clarke D.F.5; Hynes M.J.5; Davis M.A.5; Todd R.B.4
2014
Source PublicationEukaryotic Cell
ISSN15359778
Volume13Issue:4Pages:527-538
Abstract

The Aspergillus nidulans GATA transcription factor AreA activates transcription of nitrogen metabolic genes in response to nitrogen limitation and is known to accumulate in the nucleus during nitrogen starvation. Sequence analysis of AreA revealed multiple nuclear localization signals (NLSs), five putative classical NLSs conserved in fungal AreA orthologs but not in the Saccharomyces cerevisiae functional orthologs Gln3p and Gat1p, and one putative noncanonical RRXRXR bipartite NLS within the DNA-binding domain. In order to identify the functional NLSs in AreA, we constructed areA mutants with mutations in individual putative NLSs or combinations of putative NLSs and strains expressing green fluorescent protein (GFP)-AreA NLS fusion genes. Deletion of all five classical NLSs individually or collectively did not affect utilization of nitrogen sources or AreAdependent gene expression and did not prevent AreA nuclear localization. Mutation of the bipartite NLS conferred the inability to utilize alternative nitrogen sources and abolished AreA-dependent gene expression likely due to effects on DNA binding but did not prevent AreA nuclear localization. Mutation of all six NLSs simultaneously prevented AreA nuclear accumulation. The bipartite NLS alone strongly directed GFP to the nucleus, whereas the classical NLSs collaborated to direct GFP to the nucleus. Therefore, AreA contains multiple conserved NLSs, which show redundancy and together function to mediate nuclear import. The noncanonical bipartite NLS is conserved in GATA factors from Aspergillus, yeast, and mammals, indicating an ancient origin. © 2014, American Society for Microbiology. All Rights Reserved.

DOI10.1128/EC.00040-14
URLView the original
Indexed BySCI
Language英语
WOS Research AreaMicrobiology ; Mycology
WOS SubjectMicrobiology ; Mycology
WOS IDWOS:000333903000009
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Cited Times [WOS]:15   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Affiliation1.Rand Farm Supplies
2.Universidade de Macau
3.University of Queensland
4.Kansas State University
5.University of Melbourne
6.Commonwealth Scientific and Industrial Research Organization
Recommended Citation
GB/T 7714
Hunter C.C.,Siebert K.S.,Downes D.J.,et al. Multiple nuclear localization signals mediate nuclear localization of the GATA transcription factor AreA[J]. Eukaryotic Cell,2014,13(4):527-538.
APA Hunter C.C..,Siebert K.S..,Downes D.J..,Wong K.H..,Kreutzberger S.D..,...&Todd R.B..(2014).Multiple nuclear localization signals mediate nuclear localization of the GATA transcription factor AreA.Eukaryotic Cell,13(4),527-538.
MLA Hunter C.C.,et al."Multiple nuclear localization signals mediate nuclear localization of the GATA transcription factor AreA".Eukaryotic Cell 13.4(2014):527-538.
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