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Hsf1 and Hsp90 orchestrate temperature-dependent global transcriptional remodelling and chromatin architecture in Candida albicans
Michelle D. Leach1,2; Rhys A. Farrer3; Kaeling Tan4; Zhengqiang Miao4; Louise A. Walker5; Christina A. Cuomo3; Robert T. Wheeler5; Alistair J. P. Brown1; Koon Ho Wong4; Leah E. Cowen2
2016-05-26
Source PublicationNature Communications
ISSN20411723
Volume7
Abstract

Fever is a universal response to infection, and opportunistic pathogens such as Candida albicans have evolved complex circuitry to sense and respond to heat. Here we harness RNA-seq and ChIP-seq to discover that the heat shock transcription factor, Hsf1, binds distinct motifs in nucleosome-depleted promoter regions to regulate heat shock genes and genes involved in virulence in C. albicans. Consequently, heat shock increases C. albicans host cell adhesion, damage and virulence. Hsf1 activation depends upon the molecular chaperone Hsp90 under basal and heat shock conditions, but the effects are opposite and in part controlled at the level of Hsf1 expression and DNA binding. Finally, we demonstrate that Hsp90 regulates global transcription programs by modulating nucleosome levels at promoters of stress-responsive genes. Thus, we describe a mechanism by which C. albicans responds to temperature via Hsf1 and Hsp90 to orchestrate gene expression and chromatin architecture, thereby enabling thermal adaptation and virulence.

DOI10.1038/ncomms11704
URLView the original
Indexed BySCIE
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000376671100001
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Cited Times [WOS]:34   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Corresponding AuthorLeah E. Cowen
Affiliation1.Aberdeen Fungal Group, University of Aberdeen, Institute of Medical Sciences, Foresterhill, AB25 2ZD, Aberdeen, UK
2.Department of Molecular Genetics, University of Toronto, Toronto, M5S 1A8, Ontario, Canada
3.Genome Sequencing and Analysis Program, Broad Institute of MIT and Harvard, Cambridge, 02142, Massachusetts, USA
4.Faculty of Health Sciences, University of Macau, Macau, 999078, SAR, China
5.Department of Molecular and Biomedical Sciences, University of Maine, Orono, 04469, Maine, USA
Recommended Citation
GB/T 7714
Michelle D. Leach,Rhys A. Farrer,Kaeling Tan,et al. Hsf1 and Hsp90 orchestrate temperature-dependent global transcriptional remodelling and chromatin architecture in Candida albicans[J]. Nature Communications,2016,7.
APA Michelle D. Leach.,Rhys A. Farrer.,Kaeling Tan.,Zhengqiang Miao.,Louise A. Walker.,...&Leah E. Cowen.(2016).Hsf1 and Hsp90 orchestrate temperature-dependent global transcriptional remodelling and chromatin architecture in Candida albicans.Nature Communications,7.
MLA Michelle D. Leach,et al."Hsf1 and Hsp90 orchestrate temperature-dependent global transcriptional remodelling and chromatin architecture in Candida albicans".Nature Communications 7(2016).
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