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Thermo-responsive fluorescent polymers with diverse LCSTs for ratiometric temperature sensing through FRET
Ding Z.; Wang C.; Feng G.; Zhang X.
2018-03-08
Source PublicationPolymers
ISSN20734360
Volume10Issue:3
Abstract

Temperature is a significant parameter to regulate biological reactions and functions inside cells. Sensing the intracellular temperature with a competent method is necessary to understand life science. In this work, an energy-transfer polymeric thermometer was designed for temperature sensing. The thermometer was prepared from two thermo-responsive polymers with different lower critical solution temperatures (LCSTs) of 31.1 °C and 48.6 °C, coupling with blue and red fluorescent molecules, respectively, developed for ratiometric temperature sensing based on the Förster resonance energy transfer (FRET) mechanism. The polymers were synthesized from two monomers, N-isopropylacrylamide (NIPA) and N-isopropylmethacrylamide (NIPmA), which provided different temperature responses. The fluorescent intensity of each polymer (peaked at 436 and 628 nm, respectively) decreased upon the heating of the polymer aqueous solution. While these two polymer aqueous solutions were mixed, the fluorescent intensity decrease at 436 nm and substantial fluorescence enhancement at 628 nm was observed with the increasing temperature due to FRET effect. The cell imaging of HeLa cells by these thermo-responsive polymers was explored. The difference of LCSTs resulting in ratiometric fluorescence change would have a potential impact on the various biomedical applications.

KeywordFret N-isopropylacrylamide N-isopropylmethacrylamide Ratiometric Temperature Sensing
DOI10.3390/polym10030283
URLView the original
Indexed BySCI
Language英语
WOS Research AreaPolymer Science
WOS SubjectPolymer Science
WOS IDWOS:Polymer Science
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Document TypeJournal article
CollectionFaculty of Health Sciences
AffiliationUniversidade de Macau
Recommended Citation
GB/T 7714
Ding Z.,Wang C.,Feng G.,et al. Thermo-responsive fluorescent polymers with diverse LCSTs for ratiometric temperature sensing through FRET[J]. Polymers,2018,10(3).
APA Ding Z.,Wang C.,Feng G.,&Zhang X..(2018).Thermo-responsive fluorescent polymers with diverse LCSTs for ratiometric temperature sensing through FRET.Polymers,10(3).
MLA Ding Z.,et al."Thermo-responsive fluorescent polymers with diverse LCSTs for ratiometric temperature sensing through FRET".Polymers 10.3(2018).
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