UM  > 健康科學學院
Construction of ratiometric fluorescent probe based on inverse electron-demand Diels–Alder reaction for pH measurement in living cells
Feng G.; Zhang B.; Wang C.; Tan J.; Wang S.; Ding Z.; Zhang X.
2018-12-20
Source PublicationSensors and Actuators, B: Chemical
ISSN09254005
Volume277Pages:320-327
Abstract

To endow fluorescent functions to the inverse electron demand Diels–Alder (IEDDA) reaction products, introduction of extraneous fluorophores is usually inevitable. However, this strategy is blamed for complex construction and background fluorescence. It is desirable to construct IEDDA fluorescent products in a more convenient manner. In this work, we reported the in-situ generated green fluorescence in IEDDA reaction. The fluorescence intensity of IEDDA products RA-TZ-1 and RA-TZ-2 decreased in reduced pH values (from pH 7.0–3.0). Based on this novel property, we developed a ratiometric fluorescent probe RB–RA-TZ-2 by conjugation with another probe RB-NH (Em = 584 nm) with inverse response toward pH values. By single excitation, the fluorescence intensity ratio (I/I) showed a linear response (R = 0.9797) to H in pH range of 3.5–5.0. Colocalization imaging with LysoTracker Green indicated the capacity of RB–RA-TZ-2 to detect lysosomal pH (Pearson's coefficient 0.81). Confocal microscopy was applied to measure and image intracellular pH in living cells.

KeywordIn Situ Fluorescence Inverse Electron Demand Diels–alder Reaction Ph Probe Ratiometric Analysis
DOI10.1016/j.snb.2018.09.022
URLView the original
Indexed BySCI
Language英语
WOS Research AreaChemistry ; Electrochemistry ; Instruments & Instrumentation
WOS SubjectChemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation
WOS IDWOS:000453066700040
Fulltext Access
Citation statistics
Document TypeJournal article
CollectionFaculty of Health Sciences
AffiliationUniversidade de Macau
Recommended Citation
GB/T 7714
Feng G.,Zhang B.,Wang C.,et al. Construction of ratiometric fluorescent probe based on inverse electron-demand Diels–Alder reaction for pH measurement in living cells[J]. Sensors and Actuators, B: Chemical,2018,277:320-327.
APA Feng G..,Zhang B..,Wang C..,Tan J..,Wang S..,...&Zhang X..(2018).Construction of ratiometric fluorescent probe based on inverse electron-demand Diels–Alder reaction for pH measurement in living cells.Sensors and Actuators, B: Chemical,277,320-327.
MLA Feng G.,et al."Construction of ratiometric fluorescent probe based on inverse electron-demand Diels–Alder reaction for pH measurement in living cells".Sensors and Actuators, B: Chemical 277(2018):320-327.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Feng G.]'s Articles
[Zhang B.]'s Articles
[Wang C.]'s Articles
Baidu academic
Similar articles in Baidu academic
[Feng G.]'s Articles
[Zhang B.]'s Articles
[Wang C.]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Feng G.]'s Articles
[Zhang B.]'s Articles
[Wang C.]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.