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Generation of anti-idiotype scFv for pharmacokinetic measurement in lymphoma patients treated with chimera anti-CD22 antibody SM03
Zhao Q.1; Wong P.-F.1; Lee S.S.T.1; Leung S.-O.3; Cheung W.-T.1; Wang J.-Z.2
2014-05-09
Source PublicationPLoS ONE
ISSN19326203
Volume9Issue:5
Abstract

Pre-clinical and clinical studies of therapeutic antibodies require highly specific reagents to examine their immune responses, bio-distributions, immunogenicity, and pharmacodynamics in patients. Selective antigen-mimicking anti-idiotype antibody facilitates the assessment of therapeutic antibody in the detection, quantitation and characterization of antibody immune responses. Using mouse specific degenerate primer pairs and splenocytic RNA, we generated an idiotype antibody-immunized phage-displayed scFv library in which an anti-idiotype antibody against the therapeutic chimera anti-CD22 antibody SM03 was isolated. The anti-idiotype scFv recognized the idiotype of anti-CD22 antibody and inhibited binding of SM03 to CD22 on Raji cell surface. The anti-idiotype scFv was subsequently classified as Ab2c type. Moreover, our results also demonstrated firstly that the anti-idiotype scFv could be used for pharmacokinetic measurement of circulating residual antibody in lymphoma patients treated with chimera anti-CD22 monoclonal antibody SM03. Of important, the present approach could be easily adopted to generate anti-idiotype antibodies for therapeutic antibodies targeting membrane proteins, saving the cost and time for producing a soluble antigen. © 2014 Zhao et al.

DOI10.1371/journal.pone.0096697
URLView the original
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000336838000052
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Cited Times [WOS]:1   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Affiliation1.Chinese University of Hong Kong
2.National Institutes for Food and Drug Control
3.Fudan University
Recommended Citation
GB/T 7714
Zhao Q.,Wong P.-F.,Lee S.S.T.,et al. Generation of anti-idiotype scFv for pharmacokinetic measurement in lymphoma patients treated with chimera anti-CD22 antibody SM03[J]. PLoS ONE,2014,9(5).
APA Zhao Q.,Wong P.-F.,Lee S.S.T.,Leung S.-O.,Cheung W.-T.,&Wang J.-Z..(2014).Generation of anti-idiotype scFv for pharmacokinetic measurement in lymphoma patients treated with chimera anti-CD22 antibody SM03.PLoS ONE,9(5).
MLA Zhao Q.,et al."Generation of anti-idiotype scFv for pharmacokinetic measurement in lymphoma patients treated with chimera anti-CD22 antibody SM03".PLoS ONE 9.5(2014).
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