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Scalable Generation of Mesenchymal Stem Cells from Human Embryonic Stem Cells in 3D
Yan, Li1; Jiang, Bin1; Li, Enqin1; Wang, Xiaoyan1; Ling, Qinjie2; Zheng, Dejin1; Park, Jung Woo1; Chen, Xin1; Cheung, Edwin1; Du, Xin3; Li, Yingcui4; Cheng, Gregory1; He, Erxing2; Xu, Ren-He1

Human embryonic stem cell (hESC) derived mesenchymal stem cells (EMSC) are efficacious in treating a series of autoimmune, inflammatory, and degenerative diseases in animal models. However, all the EMSC derivation methods reported so far rely on two-dimensional (2D) culture systems, which are inefficient, costive and difficult for large-scale production. HESC, as an unlimited source, can be successively propagated in spheroids. Here, we demonstrate that hESC spheroids can directly differentiate into MSC spheroids (EMSCsp) within 20 days in one vessel without passaging and the system is scalable to any desired size. EMSCsp can further differentiate into osteocytes and chondrocytes in spheres or demineralized bone matrix. EMSCsp also retains immune-modulatory effects in vitro and therapeutic effects on two mouse models of colitis after dissociation. Compared to EMSC differentiated in monolayer, EMSCsp-derived cells have faster proliferation and higher yield and develop less apoptosis and slower senescence. Thus, the 3D differentiation system allows simple, cost-effective, and scalable production of high-quality EMSC and subsequently bone and cartilage tissues for therapeutic application.

KeywordMesenchymal Stem Cells Human Pluripotent Stem Cells 3d Spheroids Colitis
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Indexed BySCI
WOS Research AreaBiochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics
WOS SubjectBiochemistry & Molecular Biology ; Biology
WOS IDWOS:000441909000005
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Cited Times [WOS]:14   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Institute of Chinese Medical Sciences
Affiliation1.Faculty of Health Sciences, University of Macau, Taipa, Macau, China
2.Department of Orthopedics, First Affiliated Hospital, Guangzhou Medical University, Guangzhou, Guangdong, China
3.Department of Hematology, Guangdong General Hospital, Guangzhou, Guangdong, China
4.Department of Biology, University of Hartford, West Hartford, Connecticut, USA
First Author AffilicationFaculty of Health Sciences
Recommended Citation
GB/T 7714
Yan, Li,Jiang, Bin,Li, Enqin,et al. Scalable Generation of Mesenchymal Stem Cells from Human Embryonic Stem Cells in 3D[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES,2018,14(10):1196-1210.
APA Yan, Li.,Jiang, Bin.,Li, Enqin.,Wang, Xiaoyan.,Ling, Qinjie.,...&Xu, Ren-He.(2018).Scalable Generation of Mesenchymal Stem Cells from Human Embryonic Stem Cells in 3D.INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES,14(10),1196-1210.
MLA Yan, Li,et al."Scalable Generation of Mesenchymal Stem Cells from Human Embryonic Stem Cells in 3D".INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES 14.10(2018):1196-1210.
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